The object of this study was to evaluate the effect of the number of cyclic loading and materials for abutment on the screw loosening. Three different abutments were tested such as titanium, zirconia and zirconia-alumina composite. Titanium fixtures and screws were used for implant systems for all experimental groups. Four different cyclic loads (105, 5×105, 10×105 a nd 20×1 05), between 0 and 60 N, were applied 30° angled to the axial direction of the underlying abutment. Removal torque of screw was measured after and before cyclic loading using digital torque gauge. Five measurements were performed at each experimental group. The data were analyzed using t-test and Tukey multiple comparisons test (p=0.05). Although there was a significant decrease in the removal torque values in the all of experimental groups, screw loosening could not be detected tactilely. This may indicate that the remaining tightening torque would serve clinically for a longer period of time regardless of abutment materials as well as application of cyclic loading.