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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Journal of the Korean Surgical Society Vol.85 No.6
- 발행연도
- 2013.12
- 수록면
- 249 - 260 (12page)
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초록· 키워드
오류제보하기
Purpose: To investigate the chemotherapeutic effect of quercetin against cancer cells, signaling pathway of apoptosis was explored in human pancreatic cells.
Methods: Various anticancer drugs including adriamycin, cisplatin, 5-fluorouracil (5-FU) and gemcitabine were used. Cell viability was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphe-nyltetra zolium bromide assay. Apoptosis was determined by 4’-6-diamidino-2-phenylindole nuclei staining and flow cytometry in PANC-1 cells treated with 50 μg/mL quercetin for 24 hours. Expression of endoplas mic reticulum (ER) stress mediators including, Grp78/Bip, p-PERK, PERK, ATF4, ATF6 and GADD153/CHOP proteins were measured by Western blot analysis. Mitochondrial membrane potential was measured by fluorescence staining with JC-1, rhodamine 123. Quercetin induced the apoptosis of PANC-1, which was characterized as nucleic acid and genomic DNA fragmentation, chromatin condensation, and sub-G0/G1 fraction of cell cycle increase. But not adriamycin, cisplatin, gemcitabine, and 5-FU. PANC-1 cells were markedly sensitive to quercetin.
Results: Treatment with quercetin resulted in the increased accumulation of intracellular Ca<SUP>2+</SUP> ion. Treatment with quercetin also increased the expression of Grp78/Bip and GADD153/CHOP protein and induced mitochondrial dysfunction. Quercetin exerted cytotoxicity against human pancreatic cancer cells via ER stressmediated apoptotic signaling including reactive oxygen species production and mitochondrial dysfunction.
Conclusion: These data suggest that quercetin may be an important modulator of chemosensitivity of cancer cells against anticancer chemotherapeutic agents.
Methods: Various anticancer drugs including adriamycin, cisplatin, 5-fluorouracil (5-FU) and gemcitabine were used. Cell viability was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphe-nyltetra zolium bromide assay. Apoptosis was determined by 4’-6-diamidino-2-phenylindole nuclei staining and flow cytometry in PANC-1 cells treated with 50 μg/mL quercetin for 24 hours. Expression of endoplas mic reticulum (ER) stress mediators including, Grp78/Bip, p-PERK, PERK, ATF4, ATF6 and GADD153/CHOP proteins were measured by Western blot analysis. Mitochondrial membrane potential was measured by fluorescence staining with JC-1, rhodamine 123. Quercetin induced the apoptosis of PANC-1, which was characterized as nucleic acid and genomic DNA fragmentation, chromatin condensation, and sub-G0/G1 fraction of cell cycle increase. But not adriamycin, cisplatin, gemcitabine, and 5-FU. PANC-1 cells were markedly sensitive to quercetin.
Results: Treatment with quercetin resulted in the increased accumulation of intracellular Ca<SUP>2+</SUP> ion. Treatment with quercetin also increased the expression of Grp78/Bip and GADD153/CHOP protein and induced mitochondrial dysfunction. Quercetin exerted cytotoxicity against human pancreatic cancer cells via ER stressmediated apoptotic signaling including reactive oxygen species production and mitochondrial dysfunction.
Conclusion: These data suggest that quercetin may be an important modulator of chemosensitivity of cancer cells against anticancer chemotherapeutic agents.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (30)
참고문헌 신청
1. [학술지(정기간행물)] - 김용태 / 2008 / 췌장암의 항암화학요법 / 대한소화기학회지 51 (2) : 111 ~ 118
2. [학술지(정기간행물)] - Ekbom A / 1994 / Pancreatitis and pancreatic cancer: a population-based study / J Natl Cancer Inst 86 : 625 ~ 627
3. [학술지(정기간행물)] - Mehmet H. / 2000 / Caspases find a new place to hide / Nature 403 : 29 ~ 30
4. [학술지(정기간행물)] - Nakagawa T / 2000 / Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta / Nature 403 : 98 ~ 103
5. [학술지(정기간행물)] - Morishima N / 2002 / An endoplasmic reticulum stress-specific caspase cascade in apoptosis: cytochrome c-independent activation of caspase-9 by caspase-12 / J Biol Chem 277 : 34287 ~ 34294
2. [학술지(정기간행물)] - Ekbom A / 1994 / Pancreatitis and pancreatic cancer: a population-based study / J Natl Cancer Inst 86 : 625 ~ 627
3. [학술지(정기간행물)] - Mehmet H. / 2000 / Caspases find a new place to hide / Nature 403 : 29 ~ 30
4. [학술지(정기간행물)] - Nakagawa T / 2000 / Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta / Nature 403 : 98 ~ 103
5. [학술지(정기간행물)] - Morishima N / 2002 / An endoplasmic reticulum stress-specific caspase cascade in apoptosis: cytochrome c-independent activation of caspase-9 by caspase-12 / J Biol Chem 277 : 34287 ~ 34294
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UCI(KEPA) : I410-ECN-0101-2015-510-000972789