식물추출물 후추 추출물과 카시아 오일, 라벤더 오일을 이용하여 친환경유기농자재로 개발 중인 제품으로 배추좀나방 방제에 이용할 수 있는 후추 추출물+카시아 오일 57.5% (후보제형 A), 후추 추출물+카시아 오일 57%(후보제형 B), 후추 추출물+라벤더 오일 50% (후보제형 C) 유제의 생태에 대한 급성독성을 평가하여 친환경농자재 시제품으로서의 활용가능성을 확인하고자 하였다. 물벼룩 급성독성을 실시한 결과 후보제형 A 및 C 유제의 EC50은 각 0.46, 0.25 mg L<SUP>?1</SUP>로 EPA 기준으로 강한 독성이었고, 후보제형 B 유제의 EC<SUB>50</SUB>는 1.9 mg L<SUP>?1</SUP>로 보통독성이었다. 잉어 급성독성 시험의 경우, 후보제형 A 유제의 LC<SUB>50</SUB>가 1.9 mg L<SUP>?1</SUP>으로 농촌진흥청 고시에 따라 어독성 II급 농약이 었으며 후보제형 B, C 유제는 2.9, 3.8 mg L<SUP>?1</SUP>로 어독성 III급 농약으로 구분되었다. 꿀벌 급성독성시험은 접촉과 섭식 시험으로 나누어서 실시하였고, 후보 3종 모두 접촉과 섭식독성 LD<SUB>50</SUB>가 100 μg a.i bee<SUP>?1</SUP> 이상으로 나타나 독성이 낮은 것으로 판단되었다. 지렁이 급성독성시험의 경우, 후보제형 A, B 및 C 유제의 LC<SUB>50</SUB>가 각각 887, 988, 564 mg kg<SUP>?1</SUP>이었다. 후추 추출물+카시아 오일 57% (후보제형 B), 후추 추출물+라벤더 오일 50% (후보제형 C) 유제는 어독성 및 꿀벌 급성독성이 낮아 친환경 농자재로서 활용이 가능할 것으로 사료되었다.
Environment-friendly agro-materials tend to be preferred to chemical insecticides recently. For this reason, many studies are conducted to develop environment-friendly insecticides containing natural materials. The purpose of this study was to assess ecotoxicity for pepper extract and cassia oil (11.5+46%, A), pepper extract and cassia oil (23+34%, B), and pepper extract and lavender oil (40+10%, C). They are expected to prevent from pests especially diamondback moth, and can be used for agro-materials. Their formulation was emusifiable concentration (EC). Target species used to assess acute toxicity were aquatic invertebrate (Daphina magna), fish (Cyprinus carpio), honeybee (Apis mellifera L.) and earthworm (Eisenia fetida). The EC<SUB>50</SUB> value of A, B, and C to aquatic invertebrate were 0.46, 1.9, 0.25 mg L<SUP>?1</SUP> respectively and these values were moderately toxic according to standard of USEPA. In case of acute toxicity test to fish, the LC<SUB>50</SUB> of A, B, and C were 1.9, 2.9, 3.8 mg L<SUP>?1</SUP> respectively. A was category II in acute toxicity of fish and not acceptable to evaluation criteria of environment-friendly agro-materials. B and C were category III and acceptable. Acute contact and oral toxicity test to honeybee were conducted and the LD<SUB>50</SUB> of A, B, and C were > 100 μg a.i. bee<SUP>?1</SUP> in both of tests. It indicated they were low toxic to honeybee. In case of acute toxicity test to earthworm, LC<SUB>50</SUB> of A, B, and C were 695, 988, and 564 mg kg<SUP>?1</SUP>. In conclusion, pepper extract+cassia oil 57% EC and pepper extract+lavender oil 50% EC were expected to be used for environment-friendly insecticide materials with low risk against ecosystem and contribute to developing environment-friendly agro-materials.