지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.9
- 수록면
- 41 - 45 (5page)
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초록· 키워드
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Two-photon microscopy (TPM) is minimally-invasive 3D fluorescence microscopy based on nonlinear excitation, and TPM can visualize cellular structures based on auto-fluorescence. Line-scanning TPM is one of high-speed TPM methods without sacrificing the image resolution by using spatial and temporal focusing. In this paper, we developed line-scanning TPM based on spatial and temporal focusing for auto-fluorescence imaging by exciting the tryptophan. Laser source for this system was an optical parametric oscillator (OPO) and it made near 570 ㎚ femtosecond pulse laser. It had 200fs pulse width and 1.72 ㎚ bandwidth, so that the achievable depth resolution was 2.4l ㎛ and field of view (FOV) is 1O.8 ㎛. From the characterization, our system has 3.0 ㎛ depth resolution and 12.3 ㎛ FOV. We visualized fixed leukocyte cell sample and compared with point scanning system.
목차
Abstract
1. 서론
2. 시스템 구성 및 성능
3. 백혈구 촬영
4. 결론
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UCI(KEPA) : I410-ECN-0101-2015-500-001014273