지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.10
- 수록면
- 245 - 250 (6page)
이용수
초록· 키워드
오류제보하기
Chalkbrood and Stonebrood are well known fungal diseases of honeybee, those are caused by the fungus Ascosphaera apis and by several species of Aspergillus, respectively. Because there is no escaped, the rapid detection system using fiend will be important in hive management. In this study a specific loop-mediated isothermal amplification detection method to A. Apis and A. flavus (referred as A. apis & A. flavus-LAMP) was developed for easy and rapid detection of the A. apis and A. flavus that cause fungal diseases in honeybee. A. apis & A. flavus-LAMP employs Bst DNA polymerase and a set of four specially designed primers named A. apis & A. flavus-LAMP F3/B3/FIP/RIP, which recognized a total of six distinct sequences in fungal diseases (A. apis & A. flavus). In the results, the gene of the pathogen, A. apis and A. flavus, was successfully and specifically detected under isothermal conditions at about 48°C whthin 1 hour. A. apis & A. flavus-LAMP assay not only is able to be used for the detection of the pathogen, A. apis and A. flavus, of fungal diseases in the field but also could be applied to the develop of field detection methods for various viral pathogens of animals.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
LITERATURE CITED
참고문헌 (13)
참고문헌 신청
1. [학술대회논문] - Alexandra-Maria Klein / 2007 / Importance of pollinators in changing landscapes for world crops / Proc Biol Sci 274 (1608) : 303 ~ 313
2. [학술지(정기간행물)] - Allen, M.F. / 1996 / The incidence and world distribution of honey bee viruses / Bee World 77 : 141 ~ 162
3. [학술지(정기간행물)] - 조용호 / 2007 / Loop-mediated Isothermal Amplification (LAMP)을 이용한 꿀벌의 Black Queen Cell Virus (BQCV) 신속 검출법의 개발 / Journal of Apiculture 22 (2) : 139 ~ 4
4. [학술지(정기간행물)] - 이혜민 / 2004 / 꿀벌 진균성 질병의 신속 확인을 위한 Ascosphera apis, Aspergillus favus의 PCR 검출법 / Journal of Apiculture 19 (2) : 139 ~ 148
5. [학술지(정기간행물)] - Nagamine, K. / 2002 / Accelerated reaction by loop-mediated isothermal amplification using loop primers / Molecular and Cellular Probes 16 (3) : 223 ~ 229
2. [학술지(정기간행물)] - Allen, M.F. / 1996 / The incidence and world distribution of honey bee viruses / Bee World 77 : 141 ~ 162
3. [학술지(정기간행물)] - 조용호 / 2007 / Loop-mediated Isothermal Amplification (LAMP)을 이용한 꿀벌의 Black Queen Cell Virus (BQCV) 신속 검출법의 개발 / Journal of Apiculture 22 (2) : 139 ~ 4
4. [학술지(정기간행물)] - 이혜민 / 2004 / 꿀벌 진균성 질병의 신속 확인을 위한 Ascosphera apis, Aspergillus favus의 PCR 검출법 / Journal of Apiculture 19 (2) : 139 ~ 148
5. [학술지(정기간행물)] - Nagamine, K. / 2002 / Accelerated reaction by loop-mediated isothermal amplification using loop primers / Molecular and Cellular Probes 16 (3) : 223 ~ 229
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UCI(KEPA) : I410-ECN-0101-2014-520-003393133