Purpose: This study examines the relationships between academic self-efficacy, major satisfaction, and career attitude maturity among nursing students. The study further explored the mediating effect of major satisfaction on the relationship between academic self-efficacy and career attitude maturity. Methods: Data were collected via questionnaires from 239 nursing students in D city and P city from March 1 to March 30, 2012. Pearson"s correlation and multiple regression analyses were used to assess the relationships between the variables. Results: 1. The mean scores for academic self-efficacy, career attitude maturity, and major satisfaction were 3.81, 3.67, and 3.89, respectively. 2. Academic self-efficacy was positively correlated with major satisfaction and career attitude maturity. 3. Major satisfaction had a mediating effect on the relationship between academic self-efficacy and career attitude maturity. Conclusion: Academic self-efficacy is expected to serve as a foundation for the promotion of career attitude maturity.