지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.10
- 수록면
- 401 - 412 (12page)
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초록· 키워드
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Korean Red Ginseng (KRG) is an oriental herbal preparation obtained from Panax ginseng Meyer (Araliaceae). To expand our understanding of the action of KRG on central nervous system (CNS) function, we examined the effects of KRG on tissue plasminogen activator (tPA)/plasminogen activator inhibitor-1 (PAI-1) expression in rat primary astrocytes. KRG extract was treated in cultured rat primary astrocytes and neuron in a concentration range of 0.1 to 1.0 mg/mL and the expression of functional tPA/PAI-1 was examined by casein zymography, Western blot and reverse transcription-polymerase chain reaction. KRG extracts increased PAI-1 expression in rat primary astrocytes in a concentration dependent manner (0.1 to 1.0 mg/mL) without affecting the expression of tPA itself. Treatment of 1.0 mg/mL KRG increased PAI-1 protein expression in rat primary astrocytes to 319.3±65.9% as compared with control. The increased PAI-1 expression mediated the overall decrease in tPA activity in rat primary astrocytes. Due to the lack of PAI-1 expression in neuron, KRG did not affect tPA activity in neuron. KRG treatment induced a concentration dependent activation of PI3K, p38, ERK1/2, and JNK in rat primary astrocytes and treatment of PI3K or MAPK inhibitors such as LY294002, U0126, SB203580, and SP600125 (10 μM each), significantly inhibited 1.0 mg/mL KRG-induced expression of PAI-1 and down-regulation of tPA activity in rat primary astrocytes. Furthermore, compound K but not other ginsenosides such as Rb1 and Rg1 induced PAI-1 expression. KRG-induced up-regulation of PAI-1 in astrocytes may play important role in the regulation of overall tPA activity in brain, which might underlie some of the beneficial effects of KRG on CNS such as neuroprotection in ischemia and brain damaging condition as well as prevention or recovery from addiction.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (38)
참고문헌 신청
1. [학술지(정기간행물)] - Sofroniew MV / 2010 / Astrocytes: biology and pathology / Acta Neuropathol 119 : 7 ~ 35
2. [학술지(정기간행물)] - Yepes M / 2004 / New functions for an old enzyme: nonhemostatic roles for tissue-type plasminogen activator in the central nervous system / Exp Biol Med (Maywood) 229 : 1097 ~ 1104
3. [학술지(정기간행물)] - Samson AL / 2006 / Tissue-type plasminogen activator: a multifaceted modulator of neurotransmission and synaptic plasticity / Neuron 50 : 673 ~ 678
4. [학술지(정기간행물)] - Lemarchant S / 2012 / tPA in the injured central nervous system: different scenarios starring the same actor? / Neuropharmacology 62 : 749 ~ 756
5. [학술지(정기간행물)] - Kim JW / 2011 / Biphasic regulation of tissue plasminogen activator activity in ischemic rat brain and in cultured neural cells: essential role of astrocyte-derived plasminogen activator inhibitor-1 / Neurochem Int 58 : 423 ~ 433
2. [학술지(정기간행물)] - Yepes M / 2004 / New functions for an old enzyme: nonhemostatic roles for tissue-type plasminogen activator in the central nervous system / Exp Biol Med (Maywood) 229 : 1097 ~ 1104
3. [학술지(정기간행물)] - Samson AL / 2006 / Tissue-type plasminogen activator: a multifaceted modulator of neurotransmission and synaptic plasticity / Neuron 50 : 673 ~ 678
4. [학술지(정기간행물)] - Lemarchant S / 2012 / tPA in the injured central nervous system: different scenarios starring the same actor? / Neuropharmacology 62 : 749 ~ 756
5. [학술지(정기간행물)] - Kim JW / 2011 / Biphasic regulation of tissue plasminogen activator activity in ischemic rat brain and in cultured neural cells: essential role of astrocyte-derived plasminogen activator inhibitor-1 / Neurochem Int 58 : 423 ~ 433
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UCI(KEPA) : I410-ECN-0101-2014-520-002777895