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자료유형
학술저널
저자정보
선지윤 (부산대학교) 변임규 (부산대학교) 이태호 (부산대학교) 박태주 (부산대학교)
저널정보
한국생물공학회 KSBB Journal KSBB Journal 제25권 제1호
발행연도
2010.2
수록면
85 - 90 (6page)

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An ion selective microelectrode (ISME) was fabricated to measure concentrations of ammonium (NH<SUB>4+</SUB>-N) and nitrate (NO<SUB>3-</SUB>-N) according to the depth of nitrifying biofilm. The limits of detectability and validity of results were investigated to evaluate the ISME. The electromotive force (EMF) was directly proportional to the ion concentration, and average detection limits of NH<SUB>4+</SUB> and NO<SUB>3-</SUB> ISME were 10<SUP>-5.14</SUP> and 10<SUP>-5.18</SUP> M, respectively. The concentrations of NH<SUB>4+</SUB>-N and NO<SUB>3-</SUB>-N in various depths on the nitrifying biofilm were measured by the ISME. When a modified Ludzack-Ettinger (MLE) process was operated at an HRT of 6 h, concentration gradients of NH<SUB>4+</SUB>-N in the bulk solution and biofilm at depths of 100 μm decreased by 70 μM, while NO<SUB>3-</SUB>-N increased by 101 μM and remained constant thereafter. At an HRT of 4 h, concentration gradients of NH<SUB>4+</SUB>-N at depths of 500 μm decreased by 160 μM and NO<SUB>3-</SUB>-N increased by 162 μM and remained constant thereafter. As HRT decreased, the concentration gradients of NH<SUB>4+</SUB>-N and NO<SUB>3-</SUB>-N between bulk solution and biofilm was higher due to the increase of nitrogen load. Also, the concentration gradients of the NH<SUB>4+</SUB>-N and NO<SUB>3-</SUB>-N of biofilm in the second aerobic tank were lower than those of the first aerobic tank, suggesting differences of nitrogen load and concentrations of DO between the first and second aerobic tank.

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