지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.4
- 수록면
- 107 - 111 (5page)
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초록· 키워드
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The essential operating parameters in virus vaccine production are multiplicity of infection (MOI), harvest time, and infection time. Stimulating agents also can be applied in order to improve vaccine productivity further. We investigated the optimum operating conditions in porcine transmissible gastroenteritis virus (TGEV) vaccine production and the applicability of sodium butyrate (NaBu) as a stimulating agents for the improvement of vaccine productivity. The optimum MOI, infection time, and harvest time for high production of TGEV by swine testicle (ST) cells were found to be 0.0001 pfu/cell, 3 day after cell inoculation, and 24 hpi, respectively. NaBu is known as a histone deacetylase inhibitor that has been widely used for the high expression of recombinant protein using mammalian cells and for the enhancement of virus propagation. So we tried to examine the potential of NaBu as a stimulating agent and to determine the optimum concentration by comparing TGEV titers with different range of NaBu concentration. TGEV titer with 5 mM NaBu was 1.5 times higher than control. Therefore, we concluded that NaBu can be a promising agent for stimulating various vaccine production including TGEV and the optimum NaBu concentration for TGEV production was determined to be 5 mM.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
4. 결론
References
참고문헌 (17)
참고문헌 신청
장준호 / 2010 / 동물세포배양을 통한 백신 생산의 현황과 미래전망 / 한국미생물·생명공학회지 38(2):124~128
Allen, M. J / 2008 / Identification of novel small molecule enhancers of protein production by cultured mammalian cells / Biotechnol. Bioeng 100:1193~1204
Kruh, J / 1982 / Effects of sodium butyrate, a new pharmacological agent, on cells in culture / Mol. Cell. Biochem 42:65~82
김정회 / 1999 / N-acetylcysteine increases the biosynthesis of recombinant EPO in apoptotic Chinese hamster ovary cells / FREE RADICAL RESEARCH 30(2):85~91
Palermo, D. P / 1991 / Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression / J. Biotechnol 19:35~47
Allen, M. J / 2008 / Identification of novel small molecule enhancers of protein production by cultured mammalian cells / Biotechnol. Bioeng 100:1193~1204
Kruh, J / 1982 / Effects of sodium butyrate, a new pharmacological agent, on cells in culture / Mol. Cell. Biochem 42:65~82
김정회 / 1999 / N-acetylcysteine increases the biosynthesis of recombinant EPO in apoptotic Chinese hamster ovary cells / FREE RADICAL RESEARCH 30(2):85~91
Palermo, D. P / 1991 / Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression / J. Biotechnol 19:35~47
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UCI(KEPA) : I410-ECN-0101-2014-400-002396100