The purpose of this study was to provide the basic data for the exercise rehabilitation program of the patients with shoulder pain by examining the effects of pain relief and range of motion with 8 weeks of aquatic exercise program for elderly women who have shoulder pain. The range of motion was measured by flexure and extension, abduction and adduction, horizontal flexure and horizontal extension. A subjective symptom tool for shoulder pain was used, and the severity of pain was measured using VAS(Visual Analogue Scale). Aquatic exercise program was made up with warming-up, exercise, cooling down in order to 5 minutes, 40 minutes, 5 minutes and carried out during 8 weeks for 4 times a week (Mon, Tue, Thu, Fri). The strength of exercise for 1-4 weeks was 13 (a little hard) - 15 (hard), and that of 5-8 weeks was 16 (hard) - 18 (very hard). The data obtained in this experiment was processed by ‘Windows SPSS(version 20.0)’ statistical computer program, and paired sample t-test (Paired t-test) was performed in order to clarify the difference between pre-test and post-test in 8 weeks aquatic exercise program. The measurements of all the items were presented as descriptive statistics (mean ±standard error), and the significant probability of all the statistics was set to p<.05. The conclusions of this study are so follows: First, there were very significant differences in patients such as the increase of flexure and extension abduction and adduction horizontal flexure and extension in post-test than pretest and before having aquatic exercise (p<.001). Second, the patients’ subjective symptom for shoulder pain scores were decreased in the post-test than the pretest with very significant differences (p<.001). Third the patients’ awareness of pain were decreased in the post-test than the pretest with highly statistically significant differences (p<.001).