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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.10
- 수록면
- 427 - 432 (6page)
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In this study, a EXGA gene code for exo-β-1,3-glucanase from Aspergillus oryzae was overexpressed and secretory produced in Saccharomyces cerevisiae. To overexpress the β-1,3-glucanase, pGInu-exgA and pAInu-exgA plasmids having GAL10 and ADH1 promoter, respectively, and exoinulinase signal sequence (Inu s.s) were constructed and introduced in S. cerevisiae SEY2102 and 2805. The recombinant β-1,3- glucanase was successfully expressed and secreted into the medium and the β-1,3-glucanase activity in 2102/pGInu-exgA and 2102/pAInu-exgA strain were 5.01 unit/mL and 4.09 unit/mL, respectively. In the 2805/pGInu-exgA and 2805/pAInu-exgA strain, the β-1,3-glucanase activity showed 3.23 unit/mL and 3.22 unit/mL, respectively. Secretory efficiency in each strain reached 95% to 98%. Subsequently, the recombinant β-1,3-glucanase was used for ethanol production. Ethanol productivity in 2102/pAInu-exgA strain was 0.83 g/L when pre-treated Laminaria japonica which has initial reducing sugar of 1.4 g/L was used as substrate. It is assumed that the polysaccharides of Laminaria japonica was effectively saccharified by recombinant β-1,3-glucanase, resulting in increase of ethanol productivity. These results suggested that recombinant β-1,3- glucanase was efficiently overexpressed and secreted in S. cerevisiae SEY2102 as host strain by using ADH1 promoter-Inu s.s system.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
4. 결론
References
참고문헌 (25)
참고문헌 신청
이성목 / 2009 / 물리화학적 가수분해에 의한 갈조류 바이오 에탄올 생산 / 공업화학 20(5):517~521
Pitson, S. M / 1993 / Noncellulolytic fungal beta-glucanases: their physiology and regulation / Enzyme Microb. Technol 15:178~192
Guegen, Y. / 1996 / A very efficient β-glucosidase catalyst for the hydrolysis of flavor precursors of wines and fruit juices / J. Agric. Food Chem 44:2336~2340
Shoseyov, O / 1990 / Immobilized endo-β-glucosidase enriches flavor of wine and passion fruit juice / J. Agric. Food Chem 27:1973~1976
Vasserot, Y / 1995 / Monoterpenyl glycosides in plants and their biotechnological transformation / Acta. Biotechnol 15:77~95
Pitson, S. M / 1993 / Noncellulolytic fungal beta-glucanases: their physiology and regulation / Enzyme Microb. Technol 15:178~192
Guegen, Y. / 1996 / A very efficient β-glucosidase catalyst for the hydrolysis of flavor precursors of wines and fruit juices / J. Agric. Food Chem 44:2336~2340
Shoseyov, O / 1990 / Immobilized endo-β-glucosidase enriches flavor of wine and passion fruit juice / J. Agric. Food Chem 27:1973~1976
Vasserot, Y / 1995 / Monoterpenyl glycosides in plants and their biotechnological transformation / Acta. Biotechnol 15:77~95
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UCI(KEPA) : I410-ECN-0101-2014-400-002405584