지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.10
- 수록면
- 386 - 392 (7page)
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초록· 키워드
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Human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig), an immunosuppressive agent, was expressed in transgenic rice cells using RAmy3D promoter and RAmy1A signal peptide for the inducible production and secretion into culture media by sugar depletion. In this study, sodium butyrate was used as a small molecular enhancer (SME) to enhance the production of hCTLA4Ig in transgenic rice cell suspension cultures. When 1 mM sodium butyrate was added in sugar-free media, relative viability was not reduced, while the productivity was improved 1.3-fold. In addition, by supplementing 87 mM sodium pyruvate as an alternative energy source during the production phase, death rate of the cells was decreased. When sodium pyruvate was not added, most cells became dead at day 6. However, by adding sodium pyruvate, 18% of viability can be maintained until day 10 and the production of hCTLA4Ig was enhanced 1.4-fold. When the combination of sodium pyruvate and sodium butyrate at optimum concentrations was added, the highest viability and hCTLA4Ig production could be obtained. The highest level of hCTLA4Ig reached up to 35 mg/L at day 10.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
4. 결론
References
참고문헌 (19)
참고문헌 신청
1. [학술지(정기간행물)] - Strohl, W. R / 2009 / Discovery and development of biopharmaceuticals / Curr. Opin. Biotechnol 20 : 668 ~ 672
2. [학술지(정기간행물)] - Gomord, V / 2005 / Biopharmaceutical production in plants: problems, solutions and opportunities / Trends Biotechnol 23 : 559 ~ 565
3. [학술지(정기간행물)] - Doran, P. M / 2006 / Foreign protein degradation and instability in plants and plant tissue cultures / Trends Biotechnol 24 : 426 ~ 432
4. [학술지(정기간행물)] - Lui, V. C. H / 2003 / Mammary gland-specific secretion of biologically active immunosuppressive agent cytotoxic-T-lymphocyte antigen 4 human immunoglobulin fusion protein (CTLA4Ig) in milk by transgenesis / J. Immunol. Methods 277 : 171 ~ 183
5. [학술지(정기간행물)] - Hellwig, S / 2004 / Plant cell cultures for the production of recombinant proteins / Nat. Biotechnol 22 : 1415 ~ 1422
2. [학술지(정기간행물)] - Gomord, V / 2005 / Biopharmaceutical production in plants: problems, solutions and opportunities / Trends Biotechnol 23 : 559 ~ 565
3. [학술지(정기간행물)] - Doran, P. M / 2006 / Foreign protein degradation and instability in plants and plant tissue cultures / Trends Biotechnol 24 : 426 ~ 432
4. [학술지(정기간행물)] - Lui, V. C. H / 2003 / Mammary gland-specific secretion of biologically active immunosuppressive agent cytotoxic-T-lymphocyte antigen 4 human immunoglobulin fusion protein (CTLA4Ig) in milk by transgenesis / J. Immunol. Methods 277 : 171 ~ 183
5. [학술지(정기간행물)] - Hellwig, S / 2004 / Plant cell cultures for the production of recombinant proteins / Nat. Biotechnol 22 : 1415 ~ 1422
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UCI(KEPA) : I410-ECN-0101-2014-400-002405519