지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.12
- 수록면
- 557 - 560 (4page)
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초록· 키워드
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It has been known that Δ5-desaturase (TAD5) in the biosynthetic pathway of long chain polyunsaturated fatty acids of Thraustochytrium aureumis responsible for the conversion of di-homo-γ-linolenic acid (C20：4) into arachidonic acid (C20：4). The genetic sequence analysis on TAD5 of Thraustochytrium aureum ATCC34304 used in this study showed that it has two amino acid changes when compared to that of Thraustochytrium aureum TAD5 first reported in 2003. Accordingly, Thraustochytrium aureum ATCC34304 TAD5 was named TAD5_1. TAD5_1-inserted methylotropic Pichia pastoris was prepared and then cultured with a precursor fatty acid, di-homo-γ-linolenic acid. GC analysis confirmed that a certain amount of the precursor fatty acid was converted into arachidonic acid. In this study, not only a recombinant Pichia pastoris with the typical activity of Δ5-desaturase which plays an essential role in the biosynthesis of LCPUFAs was successfully made but also the preparationpotential of a recombinant Pichia pastoris strain which may synthesize eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) that are important in maintaining and improving human’s brain function was proposed.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
References
참고문헌 (23)
참고문헌 신청
1. [학술지(정기간행물)] - Horrobin, D. F / 1992 / Nutritional and medical importance of gamma-linolenic acid / Prog. Lipid Res 31 : 1 ~ 8
2. [학술지(정기간행물)] - Leonard, A. E / 2000 / cDNA cloning and characterization of human Δ5-desaturase involved in the biosynthesis of arachidonic acid / Biochem. J 347 : 719 ~ 724
3. [학술지(정기간행물)] - Drexler, H / 2003 / Metabolic engineering of fatty acids for breeding of new oilseed crops: strategies, problems and first results / J. Plant Physiol 160 : 779 ~ 802
4. [학술지(정기간행물)] - Crawford, M. A. / 1997 / Are deficits of arachidonic and docosahexanoicacids responsible for the neural and vascular complications of preterm babies / Am. J. Clin. Nutri 66 : 1032 ~ 1041
5. [학술지(정기간행물)] - Uauy, R / 2006 / Nutrition in brain development and aging: role of essential fatty acids / Nutr. Rev : 24 ~ 33
2. [학술지(정기간행물)] - Leonard, A. E / 2000 / cDNA cloning and characterization of human Δ5-desaturase involved in the biosynthesis of arachidonic acid / Biochem. J 347 : 719 ~ 724
3. [학술지(정기간행물)] - Drexler, H / 2003 / Metabolic engineering of fatty acids for breeding of new oilseed crops: strategies, problems and first results / J. Plant Physiol 160 : 779 ~ 802
4. [학술지(정기간행물)] - Crawford, M. A. / 1997 / Are deficits of arachidonic and docosahexanoicacids responsible for the neural and vascular complications of preterm babies / Am. J. Clin. Nutri 66 : 1032 ~ 1041
5. [학술지(정기간행물)] - Uauy, R / 2006 / Nutrition in brain development and aging: role of essential fatty acids / Nutr. Rev : 24 ~ 33
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UCI(KEPA) : I410-ECN-0101-2014-400-002405448