지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.12
- 수록면
- 517 - 522 (6page)
이용수
초록· 키워드
오류제보하기
In our previous study, we isolated and characterized a 1-deoxynojirimycin (DNJ)-producing bacterium, Bacillus subtilis MORI, from chungkookjang, a Korean traditional food. B. subtilis MORI was subjected to γ-irradiation and the resulting bacteria were screened for increased DNJ production. A mutant was identified that produced 7.6 times more DNJ and named B. subtilis MORI 3K-85. In this study, the protein profiles of both strains were compared by one-dimensional and two-dimensional gel electrophoresis (1-DE and 2-DE, respectively) under both native and denaturing conditions. The 1-DE native-PAGE and 1-DE SDS-PAGE analyses identified 5 and 7 bands, respectively, that were found at higher concentrations in B. subtilisMORI 3K-85 than in B. subtilis MORI. Similarly, 2-DE analyses identified 20 protein spots which were found at higher concentrations in B. subtilis MORI 3K-85. The peptide mass profiles of these 20 proteins were analyzed by MALDI-TOF and compared with peptide sequences of B. subtilis and B. amyloliquefaciens in the MASCOT database. This screening suggested that three dehydrogenases, an aldolase, a synthetase, an isomerase, a reductase, and a peroxidase are elevated in B. subtilis MORI 3K-85. Based on this data, one or more of the elevated 8 enzymes might be related to the DNJ biosynthetic pathway.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
References
참고문헌 (26)
참고문헌 신청
Asano, N / 2000 / Sugar-mimic glycosidase inhibitors: natural occurrence, biological activity and prospects for therapeutic application / Tetrahedron: Asymmetry 53:1645~1680
Watson, A. A / 2001 / Polyhydroxylated alkaloids-natural occurrence and therapeutic applications / Phytochemistry 56:265~295
Schedel, M / 2008 / Regioselective Oxidation of Aminosorbitol with Gluconobacter oxydans, Key Reaction in the Industrial 1-Deoxynojirimycin Synthesis. In Biotechnology: Biotransformations II, Volume 8b, 2nd ed / Wiley-VCH Verlag GmbH :296~307
Asano, N / 1994 / N-containing sugars from Morus alba and their glycosidase inhibitory activities / Carbohyr. Res 259:243~255
Yoshikuni, Y / 1988 / Inhibition of intestinal α-glycosidase activity and postprandial hyperglycemia by moranoline and its N-alkyl derivatives / Agric. Biol. Chem 52:121~128
Watson, A. A / 2001 / Polyhydroxylated alkaloids-natural occurrence and therapeutic applications / Phytochemistry 56:265~295
Schedel, M / 2008 / Regioselective Oxidation of Aminosorbitol with Gluconobacter oxydans, Key Reaction in the Industrial 1-Deoxynojirimycin Synthesis. In Biotechnology: Biotransformations II, Volume 8b, 2nd ed / Wiley-VCH Verlag GmbH :296~307
Asano, N / 1994 / N-containing sugars from Morus alba and their glycosidase inhibitory activities / Carbohyr. Res 259:243~255
Yoshikuni, Y / 1988 / Inhibition of intestinal α-glycosidase activity and postprandial hyperglycemia by moranoline and its N-alkyl derivatives / Agric. Biol. Chem 52:121~128
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2014-400-002405377