지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.8
- 수록면
- 257 - 262 (6page)
이용수
초록· 키워드
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The gene encoding Thermus thermophilus HJ6 laccase (Tt-laccase) was cloned, sequenced, and comprised of 1,389 nucleotides encoding a protein (462 amino acids) with a predicted molecular mass of 51,049 Da. The deduced amino acid sequence of Tt-laccase showed 99.7% and 44.3% identities to the Thermus thermophilus HB27 laccase and Synechococcus sp. RS9917 laccase, respectively. Tt-laccase gene was expressed as a fusion protein with six histidine residues in E. coli Rosetta-gami (DE3) cells, and the recombinant protein was purified to homogeneity. UV-Vis spectrum analysis revealed that the enzyme has copper atoms, a type I Cu(II) and a type III binuclear Cu(II). The optimum pH for the oxidation of guaiacol was 5.0 and the optimum temperature was 90℃ The half-life of heat inactivation was about 120 min at 90℃ The enzyme reaction was inhibited by sodium azide, L-cystein, EDTA, dithiothreitol, tropolone, and kojic acid. The enzyme oxidized various known laccase substrates, its lowest K<SUB>m</SUB> value being for 4-hydroxyindole, highest kcat value for syringaldazine, and highest k<SUB>cat</SUB>/K<SUB>m</SUB> for guaiacol.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
4. 결론
References
참고문헌 (25)
참고문헌 신청
Höfer, C / 1999 / Novel enzymatic oxidation of Mn2+ to Mn3+ catalyzed by a fungal laccase / FEBS Lett 451:186~190
Larrondo, L. F / 2003 / A novel extracellular multicopper oxidase from Phanerochaete chrysosporium with ferroxidase activity / Appl. Environ. Microbiol 69:6257~6263
Sánchez-Sutil, M. C / 2007 / Differential expression of the three multicopper oxidases from Myxococcus xanthus / J. Bacteriol 189:4887~4898
Thurston, C. F / 1994 / The structure and function of fungal laccases / Microbiology 140:19~26
Claus, H / 2002 / Redox-mediated decolorization of synthetic dyes by fungal laccases / Appl. Microbiol. Biotechnol 59:672~678
Larrondo, L. F / 2003 / A novel extracellular multicopper oxidase from Phanerochaete chrysosporium with ferroxidase activity / Appl. Environ. Microbiol 69:6257~6263
Sánchez-Sutil, M. C / 2007 / Differential expression of the three multicopper oxidases from Myxococcus xanthus / J. Bacteriol 189:4887~4898
Thurston, C. F / 1994 / The structure and function of fungal laccases / Microbiology 140:19~26
Claus, H / 2002 / Redox-mediated decolorization of synthetic dyes by fungal laccases / Appl. Microbiol. Biotechnol 59:672~678
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UCI(KEPA) : I410-ECN-0101-2014-400-002403297