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자료유형
학술저널
저자정보
한지혜 (가톨릭대학교) 남민경 (가톨릭대학교) 김용희 (광운대학교) 박대욱 (가톨릭대학교) 최은하 (광운대학교) 임향숙 (가톨릭대학교)
저널정보
한국생물공학회 KSBB Journal KSBB Journal 제27권 제5호
발행연도
2012.10
수록면
308 - 312 (5page)

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이 논문의 연구 히스토리 (2)

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Argon-plasma jet (Ar-PJ) is generated by ionizing Ar gas, and the resulting Ar-PJ consists of a mixture of neutral particles, positive ions, negative electrons, and various reactive species. Although Ar-PJ has been used in various biomedical applications, little is known about the biological effects on cells located near the plasma-exposed region. Here, we investigated the effects of the Ar-PJ on actin cytoskeleton of mouse embryonic fibroblasts (MEFs) in response to indirect as well as direct exposure to Ar-PJ. This Ar-PJ was generated at 500 mL/min of flow rate and 100 V electric power by our device mainly consisting of electrodes, dielectrics, and a high-voltage power supply. Because actin cytoskeleton is the key cellular machinery involved in cellular movement and is implicated in regulation of cancer metastasis and thus resulting in a highly desirable cancer therapeutic target, we examined the actin filament architectures in Ar-PJ-treated MEFs by staining with an actin-specific phalloidin labeled with fluorescent dye. Interestingly, the Ar-PJ treatment causes destabilization of actin filament architectures in the regions indirectly exposed to Ar-PJ, but no differences in MEFs treated with Ar gas alone and in untreated cell control, indicating that this phenomenon is a specific cellular response against Ar-PJ in the live cells, which are indirectly exposed to Ar-PJ. Collectively, our study raises the possibility that Ar-PJ may have potential as anti-cancer drug effect through direct destabilization of the actin cytoskeleton.

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Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
4. 결론
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UCI(KEPA) : I410-ECN-0101-2014-400-002407755