지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.7
- 수록면
- 332 - 340 (9page)
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초록· 키워드
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In this study, gamma rays were used to irradiate embryogenic calli induced from cotyledon explants of Panax ginseng Meyer. After the embryogenic calli were irradiated, they were transferred to adventitious roots using an induction medium; next, mutated adventitious root (MAR) lines with a high frequency of adventitious root formations were selected. Two MAR lines (MAR 5-2 and MAR 5-9) from the calli treated with 50 Gy of gamma rays were cultured on an NH₄NO₃-free Murashige and Skoog medium with indole-3-butyric acid 3 mg/L. The expression of genes related to ginsenoside biosynthesis was analyzed using reverse transcription polymerase chain reaction with RNA prepared from native ginseng (NG), non-irradiated adventitious root (NAR) and 2 MAR lines. The expression of the squalene epoxidase and dammarenediol synthase genes was increased in the MAR 5-2 line, whereas the phytosterol synthase was increased in the MAR 5-9 line. The content and pattern of major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1) were analyzed in the NG, NAR, and 2 MAR lines (MAR 5-2 and MAR 5-9) using TLC and HPLC. In the TLC analysis, the ginsenoside patterns in the NG, NAR, and 2 MAR lines were similar; in contrast, the MAR 5-9 line showed strong bands of primary ginsenosides. In the HPLC analysis, compared with the NG, one new type of ginsenoside was observed in the NAR and 2 MAR lines, and another new type of ginsenoside was observed in the 2 MAR lines irradiated with gamma rays. The ginsenoside content of the MAR 5-9 line was significantly greater in comparison to the NG.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (42)
참고문헌 신청
1. [학술지(정기간행물)] - Gillis CN. / 1997 / Panax ginseng pharmacology: a nitric oxide link? / Biochem Pharmacol 54 : 1 ~ 8
2. [학술지(정기간행물)] - Keum YS / 2000 / Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng / Cancer Lett 150 : 41 ~ 48
3. [학술지(정기간행물)] - Sticher O. / 1998 / Getting to the root of ginseng / Chemtech 28 : 26 ~ 32
4. [학술지(정기간행물)] - Kim YK / 2002 / Free radical scavenging activity of red ginseng aqueous extracts / Toxicology 172 : 149 ~ 156
5. [학술지(정기간행물)] - Vogler BK / 1999 / The efficacy of ginseng: a systematic review of randomised clinical trials / Eur J Clin Pharmacol 55 : 567 ~ 575
2. [학술지(정기간행물)] - Keum YS / 2000 / Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng / Cancer Lett 150 : 41 ~ 48
3. [학술지(정기간행물)] - Sticher O. / 1998 / Getting to the root of ginseng / Chemtech 28 : 26 ~ 32
4. [학술지(정기간행물)] - Kim YK / 2002 / Free radical scavenging activity of red ginseng aqueous extracts / Toxicology 172 : 149 ~ 156
5. [학술지(정기간행물)] - Vogler BK / 1999 / The efficacy of ginseng: a systematic review of randomised clinical trials / Eur J Clin Pharmacol 55 : 567 ~ 575
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UCI(KEPA) : I410-ECN-0101-2014-520-002962183