지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
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The tuberculin skin test (TST) and interferon gamma (IFN-γ) release assay (IGRA) have been widely used for diagnosis of latent tuberculosis infection (LTBI). In order to overcome limitations of current LTBI diagnostic methods, the development of a novel molecular assay which is able to measure the IFN-γ messenger RNA (mRNA) expression level after stimulation with Mycobacterium tuberculosis (MTB) specific antigen was recently developed. The ability of a molecular assay to detect MTB infection was similar to commercial IGRA however, the optimal incubation time for stimulating IFN-γ was not yet established. Therefore, in this study the direct comparisons of MTB Ag stimulation times (4 and 24 hrs) were performed for diagnosis of MTB infection. Data showed that the coincident rate between QFT-GIT IFN-γ ELISA and IFN-γ RT-PCR (4 hrs) was 88.35% and that of QFT-GIT and IFN-γ RT-PCR (24 hrs) was 70.85%. Based on a receiver operating characteristic (ROC) curve, the 4 hrs-MTB specific Ag stimulation time for IFN-γ RT-PCR had the significant P value, 95% CI value, and AUC (P ＜ 0.0001, 95% CI=0.82 to 1.02, and AUC=0.9214) in comparison with 24 hrs-MTB specific Ag stimulation time (P = 0.009, 95% CI=0.06 to 0.94, and AUC=0.7711). These results show that 4-hr was the most optimal MTB Ag stimulation time for performing IFN-γ RT-PCR. Although semi-quantitative RT-PCR had a few analytical limitations, it might be useful as an alternative molecular diagnostic method for detecting MTB infection.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (16)
참고문헌 신청
Bibova I / 2012 / Detection of immune cell response to M. tuberculosis-specific antigens by quantitative polymerase chain reaction / Diagn Microbiol Infect Dis 772:68~78
Comstock GW / 1974 / The prognosis of a positive tuberculin reaction in childhood and adolescence / Am J Epidemiol 99:131~138
Diel R / 2010 / Interferon gamma release assays for the diagnosis of latent M. tuberculosis infection: a systemic review and meta-analysis / Eur Respir J 37:88~99
Hanif E / 2012 / Understanding latent tuberculosis: the key to improved diagnostic and novel treatment strategies / Drug Discov Today 17:514~522
Harada N / 2008 / Comparison of the sensitivity and specificity of two whole blood interferon-gamma assays for M. tuberculosis infection / J Infect 56:348~353
Comstock GW / 1974 / The prognosis of a positive tuberculin reaction in childhood and adolescence / Am J Epidemiol 99:131~138
Diel R / 2010 / Interferon gamma release assays for the diagnosis of latent M. tuberculosis infection: a systemic review and meta-analysis / Eur Respir J 37:88~99
Hanif E / 2012 / Understanding latent tuberculosis: the key to improved diagnostic and novel treatment strategies / Drug Discov Today 17:514~522
Harada N / 2008 / Comparison of the sensitivity and specificity of two whole blood interferon-gamma assays for M. tuberculosis infection / J Infect 56:348~353
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UCI(KEPA) : I410-ECN-0101-2014-500-003262882