지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.5
- 수록면
- 53 - 59 (7page)
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이 논문의 연구 히스토리 (2)
2013
Hepatogenic differentiation of human mesenchymal stem cells from peritoneal adipose tissue
Tae Ho Hong
,
Il-Young Pa
,
k
Annals of Hepato-Biliary-Pancreatic Surgery
2013.05
학술저널
Hepatogenic differentiation of human mesenchymal stem cells from peritoneal adipose tissue
Tae Ho Hong
,
Ho Joong Choi
한국간담췌외과학회 학술대회지
2013.04
학술대회자료
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초록· 키워드
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Backgrounds/Aims: It has been reported that functional hepatogenic differentiation has the possibility to occur in subcutaneous adipose tissue-derived stem cells. However, no studies have investigated whether the adipose tissue-driven stem cells present in various body parts differ according to hepatogenic differentiations. In this study, stem cells were separated from body visceral fat and abdominal subcutaneous adipose tissue, and cultured, and then hepatogenic differentiation was induced. We aim to investigate the possibilities and aspects of hepatogenic differentiations within the two types of fat cells. Methods: Omental fat tissues were obtained as visceral fat and abdominal subcutaneous adipose tissues were obtained from patients who had suction-assisted lipectomy. Stem cells were separated from the obtained fat tissues, and then, hepatogenic differentiation was carried out by utilizing 2-step differentiation protocols. Results: After the differentiation, two types of cultured cells that showed the similar neuron-like shapes were changed to cuboidal shapes and included several binucleated cells which could be characteristics of mature hepatocytes. We confirmed that hepatocyte specific genes and proteins such as albumin and CYP3A4 were being expressed. By utilizing the ELISA test, we were able to observe that the albumin was secreted into the culture fluids in both cells. After completing the differentiation, we observed the presence of the hepatocyte specific properties by confirming glycogen storage within the cells and the ICG reagent uptake. Conclusions: We confirmed that hepatogenic differentiation was possible to occur in the omental fat as well as subcutaneous adipose tissue.
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UCI(KEPA) : I410-ECN-0101-2014-510-002512789