CD8+ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax

Hyo-Jin Kim; Bong-Kwang Jung; Jin-Joo Lee; Kyoung-Ho Pyo; Tae Yun Kim; Byung-il Choi; Tae Woo Kim; Hajime Hisaeda; Kunisuke Himeno; Eun-Hee Shin; Jong-Yil Chai

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저자정보: Hyo-Jin Kim (Seoul National University Medical Research Center) Bong-Kwang Jung (Seoul National University Medical Research Center) Jin-Joo Lee (Seoul National University Medical Research Center) Kyoung-Ho Pyo (Seoul National University Medical Research Center) Tae Yun Kim (Yonsei University College of Medicine) Byung-il Choi (Korea University) Tae Woo Kim (Korea University) Hajime Hisaeda (Gunma University) Kunisuke Himeno (Kyushu University) Eun-Hee Shin (Seoul National University Medical Research Center) Jong-Yil Chai (Seoul National University Medical Research Center)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.49 No.1

발행연도: 2011.3

수록면: 85 - 90 (6page)

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Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8+ T-cells and CD4+ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8+ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.

#Plasmodium vivax #DNA vaccine #apical membrane antigen (AMA) #CD8+ T-cell #gene gun

참고문헌 (25)

참고문헌 신청

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