지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.3
- 수록면
- 17 - 23 (7page)
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초록· 키워드
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The aim of this study was to assess the cytotoxic effects of various concentrations of miltefosine on Leishmania major (MRHO/IR/75/ER) and L. tropica (MHOM/IR/02/Mash10) promastigotes and to observe the programmed cell death features. The colorimetric MTT assay was used to find L. major and L. tropica viability and the obtained results were expressed as 50% inhibitory concentration (IC50). Also, 50% effective doses (ED50) for L. major and L. tropica amastigotes were also determined. Annexin-V FLUOS staining was performed to study the cell death properties of miltefosine using FACS analysis. Qualitative analysis of the total genomic DNA fragmentation was performed by agarose gel electrophoresis. Furthermore, to observe changes in cell morphology, promastigotes were examined using light microscopy. In both strains of L. major and L. tropica, miltefosine induced dose-dependent death with features of apoptosis, including cell shrinkage, DNA laddering, and externalization of phosphatidylserine. The IC50 was achieved at 22 μM and 11 μM for L. major and L. tropica after 48 hr of incubation, respectively. ED50 of L. major and L. tropica amastigotes were 5.7 μM and 4.2 μM, respectively. Our results indicate that miltefosine induces apoptosis of the causative agent of cutaneous leishmaniasis in a dose-dependent manner. Interestingly, L. major did not display any apoptotic changes when it was exposed to miltefosine in concentrations sufficient to kill L. tropica.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (27)
참고문헌 신청
1. [학술지(정기간행물)] - Ellis RE / 1991 / Mechanisms and functions of cell death / Ann Rev Cell Biol 7 : 663 ~ 698
2. [학술지(정기간행물)] - Raff MC / 1992 / Social controls on cell survival and cell death / Nature 356 (6368) : 397 ~ 400
3. [학술지(정기간행물)] - Vaux DL / 1993 / Toward an understanding of the molecular mechanisms of physiological cell death / Proc Natl Acad Sci USA : 786 ~ 789
4. [학술지(정기간행물)] - Steller H / 1995 / Mechanisms and genes of cellular suicide / Science 267 (5203) : 1445 ~ 1449
5. [학술지(정기간행물)] - Heussler VT / 2001 / Inhibition of apoptosis by intracellular protozoan parasites / Int J Parasitol 31 : 1166 ~ 1176
2. [학술지(정기간행물)] - Raff MC / 1992 / Social controls on cell survival and cell death / Nature 356 (6368) : 397 ~ 400
3. [학술지(정기간행물)] - Vaux DL / 1993 / Toward an understanding of the molecular mechanisms of physiological cell death / Proc Natl Acad Sci USA : 786 ~ 789
4. [학술지(정기간행물)] - Steller H / 1995 / Mechanisms and genes of cellular suicide / Science 267 (5203) : 1445 ~ 1449
5. [학술지(정기간행물)] - Heussler VT / 2001 / Inhibition of apoptosis by intracellular protozoan parasites / Int J Parasitol 31 : 1166 ~ 1176
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UCI(KEPA) : I410-ECN-0101-2014-510-002511737