지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 103 - 108 (6page)
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초록· 키워드
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Autophagy is a catabolic process involved in the degradation of a cell’s own components for cell growth, development, homeostasis, and the recycling of cellular products. Autophagosome is an essential component in the protozoan parasite during differentiation and encystation. The present study identified and characterized autophagy-related protein (Atg) 3, a member of Atg8 conjugation system, in Acanthamoeba castellanii (AcAtg3). AcAtg3 encoding a 304 amino acid protein showed high similarity with the catalytic cysteine site of other E2 like enzymes of ubiquitin system. Predicted 3D structure of AcAtg3 revealed a hammer-like shape, which is the characteristic structure of E2-like enzymes. The expression level of AcAtg3 did not increase during encystation. However, the formation of mature cysts was significantly reduced in Atg3-siRNA transfected cells in which the production of Atg8-phosphatidylethanolamine conjugate was inhibited. Fluorescent microscopic analysis revealed that dispersed AcAtg3-EGFP fusion protein gathered around autophagosomal membranes during encystation. These results provide important information for understanding autophagic machinery through the lipidation reaction mediated by Atg3 in Acanthamoeba.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (21)
참고문헌 신청
1. [단행본] - Martinez AJ / 1985 / Free-living Amoebas: Natural History, Prevention, Diagnosis, Pathology and Treatment of Disease / Boca Raton, Florida, USA. CRC Press : 5 ~ 42
2. [학술지(정기간행물)] - Picazarri K / 2008 / Autophagy during proliferation and encystation in the protozoan parasite Entamoeba invadens / Infect Immun 76 : 278 ~ 288
3. [학술지(정기간행물)] - Yorimitsu T / 2005 / Autophagy: molecular machinery for self-eating / Cell Death Differ 12 : 1542 ~ 1552
4. [학술지(정기간행물)] - Huang WP / 2002 / Autophagy in yeast: a review of the molecular machinery / Cell Struct Funct 27 : 409 ~ 420
5. [학술지(정기간행물)] - Ohsumi Y / 2004 / Two ubiquitin-like conjugation systems essential for autophagy / Semin Cell Dev Biol 15 : 231 ~ 236
2. [학술지(정기간행물)] - Picazarri K / 2008 / Autophagy during proliferation and encystation in the protozoan parasite Entamoeba invadens / Infect Immun 76 : 278 ~ 288
3. [학술지(정기간행물)] - Yorimitsu T / 2005 / Autophagy: molecular machinery for self-eating / Cell Death Differ 12 : 1542 ~ 1552
4. [학술지(정기간행물)] - Huang WP / 2002 / Autophagy in yeast: a review of the molecular machinery / Cell Struct Funct 27 : 409 ~ 420
5. [학술지(정기간행물)] - Ohsumi Y / 2004 / Two ubiquitin-like conjugation systems essential for autophagy / Semin Cell Dev Biol 15 : 231 ~ 236
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UCI(KEPA) : I410-ECN-0101-2014-510-002511535