지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.9
- 수록면
- 295 - 298 (4page)
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초록· 키워드
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Lactobacillus species in the female genital tract are thought to act as a barrier to infection. Several studies have demonstrated that lactobacilli can adhere to vaginal epithelial cells. However, little is known about how the adherence of lactobacilli to vaginal epithelial cells affects the acidity, cell viability, or proliferation of the lactobacilli themselves or those of vaginal epithelial cells. Lactobacillus acidophilus was co-cultured with immortalized human vaginal epithelial cells (MS74 cell line), and the growth of L. acidophilus and the acidity of the culture medium were measured. MS74 cell density and viability were also assessed by counting cell numbers and observing the cell attachment state. L. acidophilus showed exponential growth for the first 6 hr until 9 hr, and the pH was maintained close to 4.0-5.0 at 24 hr after culture, consistent with previous studies. The growth curve of L. acidophilus or the pH values were relatively unaffected by co-culture with MS74 cells, confirming that L. acidophilus maintains a low pH in the presence of MS74 cells. This co-culture model could therefore potentially be used to mimic vaginal conditions for future in vitro studies. On the other hand, MS74 cells co-cultured with L. acidophilus more firmly attached to the culture plate, and a higher number of cells were present compared to cells cultured in the absence of L. acidophilus. These results indicate that L. acidophilus increases MS74 cell proliferation and viability, suggesting that lactobacilli may contribute to the healthy environment for vaginal epithelial cells.
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참고문헌 (17)
참고문헌 신청
1. [학술지(정기간행물)] - Martin HL / 1999 / Vaginal lactobacilli, microbial flora, and risk of human immunodeficiency virus type 1 and sexually transmitted disease acquisition / J Infect Dis 180 : 1863 ~ 1868
2. [학술지(정기간행물)] - Torok MR / 2007 / The association between Trichomonas vaginalis infection and level of vaginal lactobacilli, in nonpregnant women / J Infect Dis 196 : 1102 ~ 1107
3. [학술지(정기간행물)] - 류재숙 / 2006 / Trichomonas vaginalis and trichomoniasis in the Republic of Korea / The Korean Journal of Parasitology 44 (2) : 101 ~ 116
4. [학술지(정기간행물)] - Chan RC / 1985 / Competitive exclusion of uropathogens from human uroepithelial cells by Lactobacillus whole cells and cell wall fragments / Infect Immun 47 : 84 ~ 89
5. [학술지(정기간행물)] - Velraeds MM / 1996 / Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates / Appl Environ Microbiol 62 : 1958 ~ 1963
2. [학술지(정기간행물)] - Torok MR / 2007 / The association between Trichomonas vaginalis infection and level of vaginal lactobacilli, in nonpregnant women / J Infect Dis 196 : 1102 ~ 1107
3. [학술지(정기간행물)] - 류재숙 / 2006 / Trichomonas vaginalis and trichomoniasis in the Republic of Korea / The Korean Journal of Parasitology 44 (2) : 101 ~ 116
4. [학술지(정기간행물)] - Chan RC / 1985 / Competitive exclusion of uropathogens from human uroepithelial cells by Lactobacillus whole cells and cell wall fragments / Infect Immun 47 : 84 ~ 89
5. [학술지(정기간행물)] - Velraeds MM / 1996 / Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates / Appl Environ Microbiol 62 : 1958 ~ 1963
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UCI(KEPA) : I410-ECN-0101-2014-510-002511444