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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.9
- 수록면
- 285 - 290 (6page)
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초록· 키워드
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Naegleria fowleri, a ubiquitous free-living ameba, causes fatal primary amebic meningoencephalitis in humans. N. fowleri trophozoites are known to induce cytopathic changes upon contact with microglial cells, including necrotic and apoptotic cell death and pro-inflammatory cytokine release. In this study, we treated rat microglial cells with amebic lysate to probe contact-independent mechanisms for cytotoxicity, determining through a combination of light microscopy and scanning and transmission electron microscopy whether N. fowleri lysate could effect on both necrosis and apoptosis on microglia in a time- as well as dose-dependent fashion. A 51Cr release assay demonstrated pronounced lysate induction of cytotoxicity (71.5%) toward microglial cells by 24 hr after its addition to cultures. In an assay of pro-inflammatory cytokine release, microglial cells treated with N. fowleri lysate produced TNF-α, IL-6, and IL-1β, though generation of the former 2 cytokines was reduced with time, and that of the last increased throughout the experimental period. In summary, N. fowleri lysate exerted strong cytopathic effects on microglial cells, and elicited pro-inflammatory cytokine release as a primary immune response.
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참고문헌 (27)
참고문헌 신청
1. [학술지(정기간행물)] - Schuster FL / 2004 / Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals / Int J Parasitol 34 : 1001 ~ 1027
2. [학술지(정기간행물)] - 신호준 / 2004 / Pathogenic free-living amoebae in Korea (Mini-Review) / The Korean Journal of Parasitology 42 (3) : 93 ~ 119
3. [학술지(정기간행물)] - John DT / 1982 / Primary amebic meningoencephalitis and the biology of Naegleria fowleri / Annu Rev Microbiol 36 : 101 ~ 123
4. [학술지(정기간행물)] - Visvesvara GS / 2007 / Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea / FEMS Immunol Med Microbiol 50 : 1 ~ 26
5. [학술지(정기간행물)] - Carter RF / 1972 / Primary amoebic meningoencephalitis: An appraisal of present knowledge / Trans R Soc Trop Med Hyg 66 : 193 ~ 213
2. [학술지(정기간행물)] - 신호준 / 2004 / Pathogenic free-living amoebae in Korea (Mini-Review) / The Korean Journal of Parasitology 42 (3) : 93 ~ 119
3. [학술지(정기간행물)] - John DT / 1982 / Primary amebic meningoencephalitis and the biology of Naegleria fowleri / Annu Rev Microbiol 36 : 101 ~ 123
4. [학술지(정기간행물)] - Visvesvara GS / 2007 / Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea / FEMS Immunol Med Microbiol 50 : 1 ~ 26
5. [학술지(정기간행물)] - Carter RF / 1972 / Primary amoebic meningoencephalitis: An appraisal of present knowledge / Trans R Soc Trop Med Hyg 66 : 193 ~ 213
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UCI(KEPA) : I410-ECN-0101-2014-510-002511424