지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.9
- 수록면
- 207 - 212 (6page)
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초록· 키워드
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Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (23)
참고문헌 신청
1. [학술지(정기간행물)] - Tenter AM / 2000 / Toxoplasma gondii: From animal to humans / Int J Pathol 30 : 1217 ~ 1258
2. [단행본] - Remington JS / 2001 / Toxoplasmosis. In Remington JS, Klein J eds, Infectious Diseases of the Fetus and Newborn Infant, 5th ed / Philadelphia, USA. WB Saunders : 205 ~ 346
3. [학술지(정기간행물)] - Omata Y / 1990 / Experimental feline toxoplasmosis: Humoral immune responses of cats inoculated orally with Toxoplasma gondii cysts and oocysts / J Vet Med Sci 52 : 865 ~ 867
4. [단행본] - Frenkel JK / 2000 / Biology of Toxoplasma gondii. In Ambroise-Thomas P, Peterse E eds, Congenital Toxoplasmosis: Scientific Background, Clinical Management and Control / Paris, France. Springer-Verlag : 9 ~ 25
5. [학술지(정기간행물)] - Wong SY / 1994 / Toxoplasmosis in pregnancy / Clin Infect Dis 18 : 853 ~ 861
2. [단행본] - Remington JS / 2001 / Toxoplasmosis. In Remington JS, Klein J eds, Infectious Diseases of the Fetus and Newborn Infant, 5th ed / Philadelphia, USA. WB Saunders : 205 ~ 346
3. [학술지(정기간행물)] - Omata Y / 1990 / Experimental feline toxoplasmosis: Humoral immune responses of cats inoculated orally with Toxoplasma gondii cysts and oocysts / J Vet Med Sci 52 : 865 ~ 867
4. [단행본] - Frenkel JK / 2000 / Biology of Toxoplasma gondii. In Ambroise-Thomas P, Peterse E eds, Congenital Toxoplasmosis: Scientific Background, Clinical Management and Control / Paris, France. Springer-Verlag : 9 ~ 25
5. [학술지(정기간행물)] - Wong SY / 1994 / Toxoplasmosis in pregnancy / Clin Infect Dis 18 : 853 ~ 861
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UCI(KEPA) : I410-ECN-0101-2014-510-002511313