Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein

Solange dos Santos Costa; Marjorie de Assis Golim; Bartira Rossi-Bergmann; Fabio Trindade Maranhão Costa; Selma Giorgio

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자료유형: 학술저널

저자정보: Solange dos Santos Costa (Universidade Estadual de Campinas) Marjorie de Assis Golim (Universidade Estadual de Campinas) Bartira Rossi-Bergmann (Universidade Estadual Paulista) Fabio Trindade Maranhão Costa (Universidade Federal do Rio de Janeiro) Selma Giorgio (Universidade Estadual de Campinas)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.49 No.4

발행연도: 2011.12

수록면: 357 - 364 (8page)

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Various Leishmania species were engineered with green fluorescent protein (GFP) using episomal vectors that encoded an antibiotic resistance gene, such as aminoglycoside geneticin sulphate (G418). Most reports of GFP-Leishmania have used the flagellated extracellular promastigote, the stage of parasite detected in the midgut of the sandfly vector; fewer studies have been performed with amastigotes, the stage of parasite detected in mammals. In this study, comparisons were made regarding the efficiency for in vitro G418 selection of GFP-Leishmania amazonensis promastigotes and amastigotes and the use of in vivo G418 selection. The GFP-promastigotes retained episomal plasmid for a prolonged period and G418 treatment was necessary and efficient for in vitro selection. In contrast, GFP-amastigotes showed low retention of the episomal plasmid in the absence of G418 selection and low sensitivity to antibiotics in vitro. The use of protocols for G418 selection using infected BALB/c mice also indicated low sensitivity to antibiotics against amastigotes in cutaneous lesions.

#Leishmania amazonensis #green fluorescent protein #macrophage #geneticin

참고문헌 (23)

참고문헌 신청

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