지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.12
- 수록면
- 349 - 356 (8page)
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The existence of symbiotic relationships between Acanthamoeba and a variety of bacteria is well-documented. However, the ability of Acanthamoeba interacting with host bacterial pathogens has gained particular attention. Here, to understand the interactions of Escherichia coli K1 and E. coli K5 strains with Acanthamoeba castellanii trophozoites and cysts, association assay, invasion assay, survival assay, and the measurement of bacterial numbers from cysts were performed, and nonpathogenic E. coli K12 was also applied. The association ratio of E. coli K1 with A. castellanii was 4.3 cfu per amoeba for 1 hr but E. coli K5 with A. castellanii was 1 cfu per amoeba for 1 hr. By invasion and survival assays, E. coli K5 was recovered less than E. coli K1 but still alive inside A. castellanii. E. coli K1 and K5 survived and multiplied intracellularly in A. castellanii. The survival assay was performed under a favourable condition for 22 hr and 43 hr with the encystment of A. castellanii. Under the favourable condition for the transformation of trophozoites into cysts, E. coli K5 multiplied significantly. Moreover, the pathogenic potential of E. coli K1 from A. castellanii cysts exhibited no changes as compared with E. coli K1 from A. castellanii trophozoites. E. coli K5 was multiplied in A. castellanii trophozoites and survived in A. castellanii cysts. Therefore, this study suggests that E. coli K5 can use A. castellanii as a reservoir host or a vector for the bacterial transmission.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (30)
참고문헌 신청
1. [학술지(정기간행물)] - Jung SY / 2007 / The capsule plays an important role in Escherichia coli K1 interactions with Acanthamoeba / Int J Parasitol 37 : 417 ~ 423
2. [학술지(정기간행물)] - Khan NA / 2006 / Acanthamoeba: biology and increasing importance in human health / FEMS Microbiol Rev 30 : 564 ~ 595
3. [학술지(정기간행물)] - Marciano-Cabral F / 2003 / Acanthamoeba spp. as agents of disease in humans / Clin Microbiol Rev 16 : 273 ~ 307
4. [학술지(정기간행물)] - Weekers PH / 1993 / Effects of grazing by the free-living soil amoebae Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis on various bacteria / Appl Environ Microbiol 59 : 2317 ~ 2319
5. [학술지(정기간행물)] - Allen PG / 1990 / Phagocytosis in Acanthamoeba: I. A mannose receptor is responsible for the binding and phagocytosis of yeast / J Cell Physiol 145 : 508 ~ 513
2. [학술지(정기간행물)] - Khan NA / 2006 / Acanthamoeba: biology and increasing importance in human health / FEMS Microbiol Rev 30 : 564 ~ 595
3. [학술지(정기간행물)] - Marciano-Cabral F / 2003 / Acanthamoeba spp. as agents of disease in humans / Clin Microbiol Rev 16 : 273 ~ 307
4. [학술지(정기간행물)] - Weekers PH / 1993 / Effects of grazing by the free-living soil amoebae Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis on various bacteria / Appl Environ Microbiol 59 : 2317 ~ 2319
5. [학술지(정기간행물)] - Allen PG / 1990 / Phagocytosis in Acanthamoeba: I. A mannose receptor is responsible for the binding and phagocytosis of yeast / J Cell Physiol 145 : 508 ~ 513
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UCI(KEPA) : I410-ECN-0101-2014-510-002511126