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Toxoplasma gondii penetrates all kinds of nucleated eukaryotic cells but modulates host cells differently for its intracellular survival. In a previous study, we found out that serine protease inhibitors B3 and B4 (SERPIN B3/B4 because of their very high homology) were significantly induced in THP-1-derived macrophages infected with T. gondii through activation of STAT6. In this study, to evaluate the effects of the induced SERPIN B3/B4 on the apoptosis of T. gondii-infected THP-1 cells, we designed and tested various small interfering (si-) RNAs of SERPIN B3 or B4 in staurosporine-induced apoptosis of THP-1 cells. Anti-apoptotic characteristics of THP-1 cells after infection with T. gondii disappeared when SERPIN B3/B4 were knock-downed with gene specific si-RNAs transfected into THP-1 cells as detected by the cleaved caspase 3, poly-ADP ribose polymerase and DNA fragmentation. This anti-apoptotic effect was confirmed in SERPIN B3/B4 overexpressed HeLa cells. We also investigated whether inhibition of STAT6 affects the function of SERPIN B3/B4, and vice versa. Inhibition of SERPIN B3/B4 did not influence STAT6 expression but SERPIN B3/B4 expression was inhibited by STAT6 si-RNA transfection, which confirmed that SERPIN B3/B4 was induced under the control of STAT6 activation. These results suggest that T. gondii induces SERPIN B3/B4 expression via STAT6 activation to inhibit the apoptosis of infected THP-1 cells for longer survival of the intracellular parasites themselves.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (25)
참고문헌 신청
Dubey JP / 2004 / Toxoplasmosis-a waterborne zoonosis / Vet Parasitol 126:57~72
Tenter AM / 2000 / Toxoplasma gondii: from animal to humans / Int J Parasitol 30:1217~1258
Montoya JG / 2004 / Toxoplasmosis / Lancet 363:1965~1976
Reiner NE / 1994 / Altered cell signaling and mononuclear phagocyte deactivation during intracellular infection / Immunol Today 15:374~381
Hall BF / 1992 / Desialylation of lysosomal membrane glycoproteins by Trypanosoma cruzi: a role for the surface neuraminidase in facilitating parasite entry into the host cell cytoplasm / J Exp Med 176:313~325
Tenter AM / 2000 / Toxoplasma gondii: from animal to humans / Int J Parasitol 30:1217~1258
Montoya JG / 2004 / Toxoplasmosis / Lancet 363:1965~1976
Reiner NE / 1994 / Altered cell signaling and mononuclear phagocyte deactivation during intracellular infection / Immunol Today 15:374~381
Hall BF / 1992 / Desialylation of lysosomal membrane glycoproteins by Trypanosoma cruzi: a role for the surface neuraminidase in facilitating parasite entry into the host cell cytoplasm / J Exp Med 176:313~325
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UCI(KEPA) : I410-ECN-0101-2014-510-002510967