지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.6
- 수록면
- 103 - 111 (9page)
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초록· 키워드
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Intestinal giant-cystic disease (IGCD) of the Israel carp (Cyprinus carpio nudus) has been recognized as one of the most serious diseases afflicting inland farmed fish in the Republic of Korea, and Thelohanellus kitauei has been identified as the causative agent of the disease. Until now, studies concerning IGCD caused by T. kitauei in the Israel carp have been limited to morphological and histopathological examinations. However, these types of diagnostic examinations are relatively time-consuming, and the infection frequently cannot be detected in its early stages. In this study, we cloned the full-length 18S rRNA gene of T. kitauei isolated from diseased Israel carps, and carried out molecular identification by comparing the sequence with those of other myxosporeans. Moreover, conventional PCR and real-time quantitative PCR (qPCR) using oligonucleotide primers for the amplification of 18S rRNA gene fragment were established for further use as methods for rapid diagnosis of IGCD. Our results demonstrated that both the conventional PCR and real-time quantitative PCR systems applied herein are effective for rapid detection of T. kitauei spores in fish tissues and environmental water.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (31)
참고문헌 신청
1. [학술지(정기간행물)] - Kitaue K / 1980 / Intestinal giant-cystic disease affecting the carp, caused by Thellohanellus sp / Fish Pathol 14 : 145 ~ 146
2. [학술지(정기간행물)] - Egusa S / 1981 / A new Myxozoa Thelohanellus kitauei, the cause of intestinal giant cystic disease of carp / Fish Pathol 15 : 213 ~ 218
3. [학술지(정기간행물)] - Chun SK / 1988 / Studies of Thelohanellus infection in carp, Cyprinus carpio. I. Experimental induction of Thelohanellosis / J Fish Pathol 1 : 111 ~ 116
4. [학술지(정기간행물)] - Rhee JK / 1990 / Prophylactic and therapeutic studies on intestinal giant-cystic disease of the Israel carp caused by Thelohanellus kitauei. I. Course of formation and vanishment of the cyst / Korean J Parasitol 28 : 183 ~ 194
5. [학술지(정기간행물)] - Rhee JK / 1990 / Prophylactic and therapeutic studies on intestinal giant-cystic disease of the Israel carp caused by Thelohanellus kitauei. II. Effects of physical and chemical factors on T. kitauei spores in vitro / Korean J Parasitol 28 : 241 ~ 252
2. [학술지(정기간행물)] - Egusa S / 1981 / A new Myxozoa Thelohanellus kitauei, the cause of intestinal giant cystic disease of carp / Fish Pathol 15 : 213 ~ 218
3. [학술지(정기간행물)] - Chun SK / 1988 / Studies of Thelohanellus infection in carp, Cyprinus carpio. I. Experimental induction of Thelohanellosis / J Fish Pathol 1 : 111 ~ 116
4. [학술지(정기간행물)] - Rhee JK / 1990 / Prophylactic and therapeutic studies on intestinal giant-cystic disease of the Israel carp caused by Thelohanellus kitauei. I. Course of formation and vanishment of the cyst / Korean J Parasitol 28 : 183 ~ 194
5. [학술지(정기간행물)] - Rhee JK / 1990 / Prophylactic and therapeutic studies on intestinal giant-cystic disease of the Israel carp caused by Thelohanellus kitauei. II. Effects of physical and chemical factors on T. kitauei spores in vitro / Korean J Parasitol 28 : 241 ~ 252
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UCI(KEPA) : I410-ECN-0101-2014-510-002510816