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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.9
- 수록면
- 243 - 247 (5page)
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초록· 키워드
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Ascaris suum eggs are inactivated by composting conditions; however, it is difficult to find functional changes in heat-treated A. suum eggs. Here, unembryonated A. suum eggs were incubated at 20˚C, 50˚C, and 70˚C in vitro, and the gene expression levels related to viability, such as eukaryotic translation initiation factor 4E (IF4E), phosphofructokinase 1 (PFK1), and thioredoxin 1 (TRX1), and to apoptosis, such as apoptosis-inducing factor 1 (AIF1) and cell death protein 6 (CDP6), were evaluated by real-time quantitative RT-PCR. No prominent morphological alterations were noted in the eggs at 20˚C until day 10. In contrast, the eggs developed rapidly, and embryonated eggs and hatched larvae began to die, starting on day 2 at 50˚C and day 1 at 70˚C. At 20˚C, IF4E, PFK1, and TRX1 mRNA expression was significantly increased from days 2-4; however, AIF1 and CDP6 mRNA expression was not changed significantly. IF4E, PFK1, and TRX1 mRNA expression was markedly decreased from day 2 at 50˚C and 70˚C, whereas AIF1 and CDP6 mRNA expression was significantly increased. The expressions of HSP70 and HSP90 were detected for 9-10 days at 20˚C, for 3-5 days at 50˚C, and for 2 days at 70˚C. Taken together, incremental heat increases were associated with the rapid development of A. suum eggs, decreased expression of genes related to viability, and earlier expression of apoptosis-related genes, and finally these changes of viability- and apoptosis-related genes of A. suum eggs were associated with survival of the eggs under temperature stress.
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참고문헌 (17)
참고문헌 신청
1. [학술지(정기간행물)] - Leles D / 2012 / Are Ascaris lumbricoides and Ascaris suum a single species? / Parasit Vectors 5 : 42 ~
2. [학술지(정기간행물)] - Collick AS / 2007 / Inactivation of Ascaris suum in a biodrying compost system / J Environ Qual 36 : 1528 ~ 1533
3. [학술지(정기간행물)] - Hamer G / 2003 / Solid waste treatment and disposal: effects on public health and environmental safety / Biotechnol Adv 22 : 71 ~ 79
4. [학술지(정기간행물)] - Pinelli E / 2009 / Prevalence of antibodies against Ascaris suum and its association with allergic manifestations in 4- year-old children in the Netherlands: The PIAMA birth cohort study / Eur J Clin Microbiol Infect Dis 28 : 1327 ~ 1334
5. [학술지(정기간행물)] - Inatomi Y / 1999 / Encephalopathy caused by visceral larva migrans due to Ascaris suum / J Neurol Sci 164 : 195 ~ 199
2. [학술지(정기간행물)] - Collick AS / 2007 / Inactivation of Ascaris suum in a biodrying compost system / J Environ Qual 36 : 1528 ~ 1533
3. [학술지(정기간행물)] - Hamer G / 2003 / Solid waste treatment and disposal: effects on public health and environmental safety / Biotechnol Adv 22 : 71 ~ 79
4. [학술지(정기간행물)] - Pinelli E / 2009 / Prevalence of antibodies against Ascaris suum and its association with allergic manifestations in 4- year-old children in the Netherlands: The PIAMA birth cohort study / Eur J Clin Microbiol Infect Dis 28 : 1327 ~ 1334
5. [학술지(정기간행물)] - Inatomi Y / 1999 / Encephalopathy caused by visceral larva migrans due to Ascaris suum / J Neurol Sci 164 : 195 ~ 199
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UCI(KEPA) : I410-ECN-0101-2014-510-002522752