Real-Time RT-PCR on SAG1 and BAG1 Gene Expression during Stage Conversion in Immunosuppressed Mice Infected with Toxoplasma gondii Tehran Strain

Monavar Selseleh; Mohammad Hossein Modarressi; Mehdi Mohebali; Saeedeh Shojaee; Mohammad Reza Eshragian; Mina Selseleh; Ebrahim Azizi; Hossein Keshavarz

추천

검색

질문

자료유형: 학술저널

저자정보: Monavar Selseleh (Tehran University of Medical Sciences) Mohammad Hossein Modarressi (Tehran University of Medical Sciences) Mehdi Mohebali (Tehran University of Medical Sciences) Saeedeh Shojaee (Tehran University of Medical Sciences) Mohammad Reza Eshragian (Tehran University of Medical Sciences) Mina Selseleh (Tehran University of Medical Sciences) Ebrahim Azizi (Tehran University of Medical Sciences) Hossein Keshavarz (Tehran University of Medical Sciences)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.50 No.3

발행연도: 2012.9

수록면: 199 - 205 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Toxoplasmic encephalitis is caused by reactivation of bradyzoites to rapidly dividing tachyzoites of the apicomplexan parasite Toxoplasma gondii in immunocompromised hosts. Diagnosis of this life-threatening disease is problematic, because it is difficult to discriminate between these 2 stages. Toxoplasma PCR assays using gDNA as a template have been unable to discriminate between an increase or decrease in SAG1 and BAG1 expression between the active tachyzoite stage and the latent bradyzoite stage. In the present study, real-time RT-PCR assay was used to detect the expression of bradyzoite (BAG1)- and tachyzoite-specific genes (SAG1) during bradyzoite/tachyzoite stage conversion in mice infected with T. gondii Tehran strain after dexamethasone sodium phosphate (DXM) administration. The conversion reaction was observed in the lungs and brain tissues of experimental mice, indicated by SAG1 expression at day 6 after DXM administration, and continued until day 14. Bradyzoites were also detected in both organs throughout the study; however, it decreased at day 14 significantly. It is suggested that during the reactivation period, bradyzoites not only escape from the cysts and reinvade neighboring cells as tachyzoites, but also converted to new bradyzoites. In summary, the real-time RT-PCR assay provided a reliable, fast, and quantitative way of detecting T. gondii reactivation in an animal model. Thus, this method may be useful for diagnosing stage conversion in clinical specimens of immunocompromised patients (HIV or transplant patients) for early identification of tachyzoite-bradyzoite stage conversion.

#Toxoplasma gondii #SAG1 #BAG1 #RT-PCR #gene expression #bradyzoite #tachyzoite

참고문헌 (31)

참고문헌 신청

1. [학술지(정기간행물)] - Mesquita RT / 2010 / Real-time quantitative PCR in cerebral toxoplasmosis diagnosis of Brazilian human immunodeficiency virus-infected patients / J Med Microbiol 59 : 641 ~ 647

2. [학술지(정기간행물)] - Cultrera R / 2001 / Expression of toxoplasmic65 kDa cystic mRNA by RT-PCR in patients with Toxoplasmagondii infection relapses / J Eukaryot Microbiol (Suppl) : 193S ~ 194S

3. [단행본] - Mariuz P / 2001 / Toxoplasma infection in HIV-infected patients, In Toxoplasmosis: A Comprehensive Clinical Guide / Cambridge University Press : 147 ~ 177

4. [학술지(정기간행물)] - Joseph P / 2002 / Optimization and evaluation of a PCR assay for detecting toxoplasmic encephalitis in patients with AIDS / J Clin Microbiol 40 : 4499 ~ 4503

5. [학술지(정기간행물)] - Sukthana Y / 2006 / Toxoplasmosis: beyond animals to humans / Trends Parasitol 22 : 137 ~ 142