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논문 기본 정보

자료유형
학술저널
저자정보
Li-Jun Jia (Yanbian University) Shou-Fa Zhang (Yanbian University) Nian-Chao Qian (Yanbian University) Xue-Nan Xuan (Obihiro University of Agriculture and Veterinary Medicine) Long-Zheng Yu (Yanbian University) Xue-Mei Zhang (Yanbian University) Ming-Ming Liu (Yanbian University)
저널정보
대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.51 No.2
발행연도
2013.4
수록면
247 - 253 (7page)

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Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. In the current study, NcSRS2 and NcGRA7 genes were spliced by overlap-extension PCR in a recombinant adenovirus termed Ad5-NcSRS2-NcGRA 7, expressing the NcSRS2-NcGRA7 gene, and the efficacy was evaluated in mice. The results showed that the titer of the recombinant adenovirus was 109TCID50/ml. Three weeks post-boost immunization (w.p.b.i.), the IgG antibody titer in sera was as high as 1:4,096. IFN-γ and IL-4 levels were significantly different from the control group (P<0.01). This research established a solid foundation for the development of a recombinant adenovirus vaccine against bovine N. caninum.

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UCI(KEPA) : I410-ECN-0101-2014-510-002535584