지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.4
- 수록면
- 147 - 154 (8page)
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초록· 키워드
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To control coccidiosis without using prophylactic medications, a DNA vaccine targeting the gametophyte antigen Gam56 from Eimeria maxima in chickens was constructed, and the immunogenicity and protective effects were evaluated. The ORF of Gam56 gene was cloned into an eukaryotic expression vector pcDNA3.1(zeo)+. Expression of Gam56 protein in COS-7 cells transfected with recombinant plasmid pcDNA-Gam56 was confirmed by indirect immunofluorescence assay. The DNA vaccine was injected intramuscularly to yellow feathered broilers of 1-week old at 3 dosages (25, 50, and 100 μg/chick). Injection was repeated once 1 week later. One week after the second injection, birds were challenged orally with 5×104 sporulated oocysts of E. maxima, then weighed and killed at day 8 post challenge. Blood samples were collected and examined for specific peripheral blood lymphocyte proliferation activity and serum antibody levels. Compared with control groups, the administration of pcDNA-Gam56 vaccine markedly increased the lymphocyte proliferation activity (P<0.05) at day 7 and 14 after the first immunization. The level of lymphocyte proliferation started to decrease on day 21 after the first immunization. A similar trend was seen in specific antibody levels. Among the 3 pcDNAGam56 immunized groups, the median dosage group displayed the highest lymphocyte proliferation and antibody levels (P<0.05). The median dosage group had the greatest relative body weight gain (89.7%), and the greatest oocyst shedding reduction (53.7%). These results indicate that median dosage of DNA vaccine had good immunogenicity and immune protection effects, and may be used in field applications for coccidiosis control.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (33)
참고문헌 신청
McDonald V / 2009 / Past and future: vaccination against Eimeria / Parasitology 136:1477~1489
Dalloul RA / 2006 / Poultry coccidiosis: recent advancements in control measures and vaccine development / Expert Rev Vaccines 5:143~163
Sharman PA / 2010 / Chasing the golden egg: vaccination against poultry coccidiosis / Parasite Immunol 32:590~598
Williams RB / 2000 / A new protocol for a challenge test to assess the efficacy of live anticoccidial vaccines for chickens / Vaccine 18:1178~1185
Dalloul RA / 2005 / Recent advances in immunomodulation and vaccination strategies against coccidiosis / Avian Dis 49:1~8
Dalloul RA / 2006 / Poultry coccidiosis: recent advancements in control measures and vaccine development / Expert Rev Vaccines 5:143~163
Sharman PA / 2010 / Chasing the golden egg: vaccination against poultry coccidiosis / Parasite Immunol 32:590~598
Williams RB / 2000 / A new protocol for a challenge test to assess the efficacy of live anticoccidial vaccines for chickens / Vaccine 18:1178~1185
Dalloul RA / 2005 / Recent advances in immunomodulation and vaccination strategies against coccidiosis / Avian Dis 49:1~8
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UCI(KEPA) : I410-ECN-0101-2014-510-002535433