지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Pluripotent stem cells (PSCs) have enormous potential in the biomedical sciences because they can grow continuously and differentiate into any kind of cell in the body. However, for future application in regenerative medicine, it is still a challenge to control the differentiation of PSCs without using genetic materials. To control the differentiation of PSCs, small molecules might be the best substitute for genetic materials considering the following advantages: small size, which enables penetration of plasma membrane; easy-to-modify structure; and low chance of genetic recombination in treated cells. Herein, we introduce small molecules that induce the neuroectodermal differentiation of mouse embryonic stem cells (ESCs). The small molecules were identified via ESC-based consecutive screenings of small-molecule libraries composed of 324 natural compounds or 93 selected drugs. The natural compounds discovered in the first screening were used to select 93 structurally similar drugs out of 1,200 approved drugs. In the second screening, among the 93 compounds, we found 4 drugs that induced the neuroectodermal differentiation of ESCs. These drugs were progesteroneor corticoid-derivatives. Our results suggest that small molecules targeting the progesterone receptor or glucocorticoid receptor could be used as chemical tools to induce the differentiation of PSCs into a specific germ lineage.
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INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (31)
참고문헌 신청
Attele AS / 1999 / Ginseng pharmacology: multiple constituents and multiple actions / Biochem Pharmacol 58:1685~1693
Borowiak M / 2009 / Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells / Cell Stem Cell 4:348~358
Dezawa M / 2001 / Specific induction of neuronal cells from bone marrow stromal cells and application for autologous transplantation / J Clin Invest 113:1701~1710
Diaz NF / 2009 / Progesterone increases dopamine neurone number in differentiating mouse embryonic stem cells / J Neuroendocrinol 21:730~736
Ding S / 2003 / Synthetic small molecules that control stem cell fate / Proc Natl Acad Sci USA 100:7632~7637
Borowiak M / 2009 / Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells / Cell Stem Cell 4:348~358
Dezawa M / 2001 / Specific induction of neuronal cells from bone marrow stromal cells and application for autologous transplantation / J Clin Invest 113:1701~1710
Diaz NF / 2009 / Progesterone increases dopamine neurone number in differentiating mouse embryonic stem cells / J Neuroendocrinol 21:730~736
Ding S / 2003 / Synthetic small molecules that control stem cell fate / Proc Natl Acad Sci USA 100:7632~7637
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UCI(KEPA) : I410-ECN-0101-2014-500-003115748