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Electrospun nanofibers are being widely used as a substratum for mammalian cell culture owing to their structural similarity to collagen fibers found in extracellular matrices of mammalian cells and tissues. Especially, development of diverse synthetic polymers has expanded use of electrospun nanofibers for constructing cell culture substrata. Synthetic polymers have several benefits comparing to natural polymer for their structural consistency, low cost, and capability for blending with other polymers or small molecules to enhance their structural integrity or add biological functions. PMGI (polymethylglutarimide) is one of the synthetic polymers that produced a rigid nanofiber that enables incorporation of small molecules, peptides, and gold nanoparticles through co-electrospinning process, during which the materials are fixed without any chemical modifications in the PMGI nanofibers by maintaining their activities. Using the phenomenon of PMGI nanofiber, here I introduce a construction method of a nanofiber substratum having cell-affinity function towards a pluripotent stem cell by incorporating a small molecule in the PMGI nanofiber.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (18)
참고문헌 신청
Dongju J / 2012 / Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells / Journal of Nanobiotechnology 10:23
Greiner A / 2007 / Electrospinning: a fascinating method for the preparation of ultrathin fibers / Angewandte Chemie International Edition English 46:5670~5703
Hansma HG / 1998 / Atomic force microscopy and other scanning probe miscroscopies / Current Opinion in Chemical Biology 2:579~584
Hoshino M / 2010 / Adhesamine, a new synthetic molecule, accelerates differentiation and prolongs survival of primary cultured mouse hippocampal neurons / Biochemical Journal 427:297~304
Khil MS / 2005 / Novel fabricated matrix via electrospinning for tissue engineering / Journal of Biomedical Materials Research B: Applied Biomaterials 72:117~124
Greiner A / 2007 / Electrospinning: a fascinating method for the preparation of ultrathin fibers / Angewandte Chemie International Edition English 46:5670~5703
Hansma HG / 1998 / Atomic force microscopy and other scanning probe miscroscopies / Current Opinion in Chemical Biology 2:579~584
Hoshino M / 2010 / Adhesamine, a new synthetic molecule, accelerates differentiation and prolongs survival of primary cultured mouse hippocampal neurons / Biochemical Journal 427:297~304
Khil MS / 2005 / Novel fabricated matrix via electrospinning for tissue engineering / Journal of Biomedical Materials Research B: Applied Biomaterials 72:117~124
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UCI(KEPA) : I410-ECN-0101-2014-500-003115733