지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.12
- 수록면
- 73 - 78 (6page)
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초록· 키워드
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Purpose: HCO₃<SUP>-</SUP> is the most important ion to buffer the acidity of saliva. The transport of HCO₃<SUP>-</SUP> is mediated by electrogenic Na<SUP>+</SUP>/HCO₃<SUP>-</SUP> cotransporter 1 (NBCe1), which expressed in various tissues including salivary glands, kidney and pancreas, etc. This experiment was performed to investigate regulatory site of NBCe1 involved in the pH regulation using various mutants of NBCe1.
Materials and Methods: Human parotid gland NBCe1 (hpNBCe1) and mutants by deletion of 1~285 bp and 1~1,035 bp were prepared. After microinjection of each cRNA to oocytes of Xenopus laevis, they were incubated for 2~3 days. The function of each protein was tested by electrophysiological method.
Results: When oocytes were exposed to the HCO₃<SUP>-</SUP> buffered solution, 1~285 bp deleted mutant hpNBCe1 evoked a marked hyperpolarization ranging from -90 ㎷ to -160 ㎷ (average: -134 ㎷; n=12) compared to the full length of hpNBCe1. Although 1~1,035 bp deleted mutant hpNBCe1 was also expressed in the plasma membrane, but it did not show any changes of membrane potentials.
Conclusion: Our deletion mutant study demonstrated that 1~285 bp of the NBCe1 is the major domain to determine HCO₃<SUP>-</SUP> transport ratio.
Materials and Methods: Human parotid gland NBCe1 (hpNBCe1) and mutants by deletion of 1~285 bp and 1~1,035 bp were prepared. After microinjection of each cRNA to oocytes of Xenopus laevis, they were incubated for 2~3 days. The function of each protein was tested by electrophysiological method.
Results: When oocytes were exposed to the HCO₃<SUP>-</SUP> buffered solution, 1~285 bp deleted mutant hpNBCe1 evoked a marked hyperpolarization ranging from -90 ㎷ to -160 ㎷ (average: -134 ㎷; n=12) compared to the full length of hpNBCe1. Although 1~1,035 bp deleted mutant hpNBCe1 was also expressed in the plasma membrane, but it did not show any changes of membrane potentials.
Conclusion: Our deletion mutant study demonstrated that 1~285 bp of the NBCe1 is the major domain to determine HCO₃<SUP>-</SUP> transport ratio.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
References
참고문헌 (19)
참고문헌 신청
1. [학술지(정기간행물)] - Helm JF / 1982 / Acid neutralizing capacity of human saliva / Gastroenterology 83 : 69 ~ 74
2. [학술지(정기간행물)] - Park K / 2002 / Expression of a sodium bicarbonate cotransporter in human parotid salivary glands / Arch Oral Biol 47 : 1 ~ 9
3. [학술지(정기간행물)] - Kim YB / 2003 / Expression of Na+/HCO3 - cotransporter and its role in pH regulation in mouse parotid acinar cells / Biochem Biophys Res Commun 304 : 593 ~ 598
4. [학술지(정기간행물)] - Romero MF / 2004 / The SLC4 family of HCO3 - transporters / Pflugers Arch 447 : 495 ~ 509
5. [학술지(정기간행물)] - 진미현 / 2008 / A role of Sodium Bicarbonate Cotransporter(NBC) in HCO3 - Formation in Human Salivary Gland Acinar Cells / International Journal of Oral Biology 33 (4) : 143 ~ 147
2. [학술지(정기간행물)] - Park K / 2002 / Expression of a sodium bicarbonate cotransporter in human parotid salivary glands / Arch Oral Biol 47 : 1 ~ 9
3. [학술지(정기간행물)] - Kim YB / 2003 / Expression of Na+/HCO3 - cotransporter and its role in pH regulation in mouse parotid acinar cells / Biochem Biophys Res Commun 304 : 593 ~ 598
4. [학술지(정기간행물)] - Romero MF / 2004 / The SLC4 family of HCO3 - transporters / Pflugers Arch 447 : 495 ~ 509
5. [학술지(정기간행물)] - 진미현 / 2008 / A role of Sodium Bicarbonate Cotransporter(NBC) in HCO3 - Formation in Human Salivary Gland Acinar Cells / International Journal of Oral Biology 33 (4) : 143 ~ 147
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UCI(KEPA) : I410-ECN-0101-2014-510-002345897