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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.12
- 수록면
- 307 - 317 (11page)
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초록· 키워드
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Objective: The purpose of this study was to investigate the isolation and characterization of multipotent human periodontal ligament (PDL) stem cells and to assess their ability to differentiate into bone, cartilage, and adipose tissue. Methods: PDL stem cells were isolated from 7 extracted human premolar teeth. Human PDL cells were expanded in culture, stained using anti-CD29, -CD34, -CD44, and -STRO-1 antibodies, and sorted by fluorescent activated cell sorting (FACS). Gingival fibroblasts (GFs) served as a positive control. PDL stem cells and GFs were cultured using standard conditions conducive for osteogenic, chondrogenic, or adipogenic differentiation. Results: An average of 152.8 ± 27.6 colony-forming units was present at day 7 in cultures of PDL stem cells. At day 4, PDL stem cells exhibited a significant increase in proliferation (p < 0.05), reaching nearly double the proliferation rate of GFs. About 5.6 ± 4.5% of cells in human PDL tissues were strongly STRO-1-positive. In osteogenic cultures, calcium nodules were observed by day 21 in PDL stem cells, which showed more intense calcium staining than GF cultures. In adipogenic cultures, both cell populations showed positive Oil Red O staining by day 21. Additionally, in chondrogenic cultures, PDL stem cells expressed collagen type II by day 21. Conclusions: The PDL contains multipotent stem cells that have the potential to differentiate into osteoblasts, chondrocytes, and adipocytes. This adult PDL stem cell population can be utilized as potential sources of PDL in tissue engineering applications.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
CONCLUSION
REFERENCES
참고문헌 (36)
참고문헌 신청
1. [학술지(정기간행물)] - Friedenstein AJ / 1970 / The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells / Cell Tissue Kinet 3 : 393 ~ 403
2. [학술지(정기간행물)] - Caplan AI / 1991 / Mesenchymal stem cells / J Orthop Res 9 : 641 ~ 650
3. [학술지(정기간행물)] - Pittenger MF / 1999 / Multilineage potential of adult human mesenchymal stem cells / Science 284 : 143 ~ 147
4. [학술지(정기간행물)] - Alhadlaq A / 2005 / Tissue-engineered osteochondral constructs in the shape of an articular condyle / J Bone Joint Surg Am 87 : 936 ~ 944
5. [학술지(정기간행물)] - Doğan A / 2003 / Healing of artificial fenestration defects by seeding of fibroblastlike cells derived from regenerated periodontal ligament in a dog: a preliminary study / Tissue Eng 9 : 1189 ~ 1196
2. [학술지(정기간행물)] - Caplan AI / 1991 / Mesenchymal stem cells / J Orthop Res 9 : 641 ~ 650
3. [학술지(정기간행물)] - Pittenger MF / 1999 / Multilineage potential of adult human mesenchymal stem cells / Science 284 : 143 ~ 147
4. [학술지(정기간행물)] - Alhadlaq A / 2005 / Tissue-engineered osteochondral constructs in the shape of an articular condyle / J Bone Joint Surg Am 87 : 936 ~ 944
5. [학술지(정기간행물)] - Doğan A / 2003 / Healing of artificial fenestration defects by seeding of fibroblastlike cells derived from regenerated periodontal ligament in a dog: a preliminary study / Tissue Eng 9 : 1189 ~ 1196
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UCI(KEPA) : I410-ECN-0101-2014-510-002357708