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논문 기본 정보

자료유형
학술대회자료
저자정보
박태현 (경남대학교) Michael C. Murphy (Louisiana State University)
저널정보
대한기계학회 대한기계학회 춘추학술대회 대한기계학회 2012년도 추계학술대회 논문집
발행연도
2012.11
수록면
640 - 643 (4page)

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초록· 키워드

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A new concept of circulating tumor cell (CTC) capture device is developed for fast, simple, and inexpensive breast cancer diagnosis. The high flow rate CTC capture device overcomes the current limitation of the CTC capture method. Current technology could not offer high flow rate capture of CTCs or high capture purity of the target cells due to the low flow velocity or lack of specific binding. The high flow rate device manipulates velocity vectors and forces the target cells toward the antibody immobilized channel surfaces to maximize the capture rate. Non-specifically bound cells washed out during rinsing process or drained through micro channels. The remained target cells, fluorescence stained and specifically bound target cells, counted using a fluorescence microscope. The high flow rate devices were hot embossed on the Poly(methyl methacrylate) and UV modified. Anti-EpCAM was immobilized on the UV modified PMMA surface after amine functionalization process. The disposable polymer CTC capture device will increase the early stage detection rate because it increase the breast cancer exam frequency. The disposable high flow rate CTC capture device shows high capture rate (~85%) at high flow rate (0.75 ㎖/min) with high purity from 7.5 ㎖ whole blood. Viable MCF-7 cells were stained with membrane fluorescence. Exact number of MCF-7 cells were spiked in 7.5 ㎖ of undiluted blood and recovered using the high flow rate device in 10 minutes.

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Abstract
1. 서론
2. 이론과개념
3. 실험방법
4. 결과및논의
5. 결론
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UCI(KEPA) : I410-ECN-0101-2014-550-000188256