지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Background : Nitric oxide (NO) is a reactive free radical gas and a messenger molecule. NO has many physiological functions, but excessive NO production induces neurotoxicity.
Objective : The present study investigated whether the aqueous extract of Polygala tenuifolia Willdenow possesses a protective effect on NO-induced apoptosis in human neuroblastoma cell line SK-N-MC.
Method : For this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-henylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and caspase-3 enzyme assay were performed.
Result : Sodium nitroprusside (SNP) exposure significantly decreased the viability of cells. The cells treated with SNP exhibited several apoptotic features such as increasing of Bax expression, caspase-3 enzyme activity and inhibiting of Bcl-2 expression. On the other hand, the viability of cells pre-treated with the aqueous extract of Polygala tenuifolia Willdenow was increased dose-dependently. The cells pre-treated for 1 h with the aqueous extract of Polygala tenuifolia Willdenow followed by treatment with SNP showed a decreased occurrence of apoptotic features like decreasing Bax expressions, caspase-3 enzyme activity and increasing Bcl-2 expressions. The aqueous extract of Polygala tenuifolia Willdenow reduced apoptotic cell death in neuroblastoma cell line SK-N-MC through the inhibition of Bax-dependent caspase-3 activation and the increasing of Bcl-2 expression.
Conclusion : Based on the present results, it is possible that Polygala tenuifolia Willdenow has therapeutic value for the treatment of a variety of NO-induced brain diseases.
Objective : The present study investigated whether the aqueous extract of Polygala tenuifolia Willdenow possesses a protective effect on NO-induced apoptosis in human neuroblastoma cell line SK-N-MC.
Method : For this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-henylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and caspase-3 enzyme assay were performed.
Result : Sodium nitroprusside (SNP) exposure significantly decreased the viability of cells. The cells treated with SNP exhibited several apoptotic features such as increasing of Bax expression, caspase-3 enzyme activity and inhibiting of Bcl-2 expression. On the other hand, the viability of cells pre-treated with the aqueous extract of Polygala tenuifolia Willdenow was increased dose-dependently. The cells pre-treated for 1 h with the aqueous extract of Polygala tenuifolia Willdenow followed by treatment with SNP showed a decreased occurrence of apoptotic features like decreasing Bax expressions, caspase-3 enzyme activity and increasing Bcl-2 expressions. The aqueous extract of Polygala tenuifolia Willdenow reduced apoptotic cell death in neuroblastoma cell line SK-N-MC through the inhibition of Bax-dependent caspase-3 activation and the increasing of Bcl-2 expression.
Conclusion : Based on the present results, it is possible that Polygala tenuifolia Willdenow has therapeutic value for the treatment of a variety of NO-induced brain diseases.
목차
Introduction
Materials and Methods
Results
Discussion
Conclusion
Acknowledgement
References
참고문헌 (28)
참고문헌 신청
1. [학술지(정기간행물)] - Ikeya Y / 2004 / Cognitive Improving and Cerebral Protective Effects of Acylated Oligosaccharides in Polygala tenuifolia / Biol Pharm Bull. 27 (7) : 1081 ~ 1085
2. [학술지(정기간행물)] - Kim You Young / 2005 / Occupational Asthma and Rhinitis Induced by a Herbal Medicine, Wonji (Polygala tenuifolia) / Journal of Korean Medical Science 20 (1) : 46 ~ 49
3. [학술지(정기간행물)] - Schmidt HH / 1994 / NO at work / Cell 78 : 919 ~ 925
4. [학술지(정기간행물)] - Yun HY / 1996 / Neurobiology of nitric oxide / Crit Rev Neurobiol 10 : 291 ~ 316
5. [학술지(정기간행물)] - Garthwaite J / 1995 / Nitric oxide signaling in the central nervous system / Annu Rev Physiol 57 : 683 ~ 706
2. [학술지(정기간행물)] - Kim You Young / 2005 / Occupational Asthma and Rhinitis Induced by a Herbal Medicine, Wonji (Polygala tenuifolia) / Journal of Korean Medical Science 20 (1) : 46 ~ 49
3. [학술지(정기간행물)] - Schmidt HH / 1994 / NO at work / Cell 78 : 919 ~ 925
4. [학술지(정기간행물)] - Yun HY / 1996 / Neurobiology of nitric oxide / Crit Rev Neurobiol 10 : 291 ~ 316
5. [학술지(정기간행물)] - Garthwaite J / 1995 / Nitric oxide signaling in the central nervous system / Annu Rev Physiol 57 : 683 ~ 706
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UCI(KEPA) : I410-ECN-0101-2014-519-000262458