지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objectives: Peroxynitrite (ONOO<SUP>-</SUP> ), superoxide anion radical (ㆍO₂<SUP>-</SUP> ) and nitric oxide (NO) are cytotoxic because they can oxidize several cellular components such as proteins, lipids and DNA. They have been implicated in the aging processes, and age-related diseases such as Alzheimer"s disease, rheumatoid arthritis, cancer, diabetes, obesity and atherosclerosis. The aim of this study was to investigate the ONOO<SUP>-</SUP> , NO, ㆍO₂<SUP>-</SUP> scavenging and NF-κB related anti-inflammatory activities of Sotosaja-hwan in ob/ob mice.
Methods: Mice were grouped and treated for 5 weeks as follows. Both the normal lean (C57/BL6J black mice) and control obese (ob/ob mice) groups have received standard chow. The experimental groups were fed with a diet of chow supplemented with 30 and 90 mg Sotosaja-hwan per 1 kg of body weight for 14 days. For this study, the fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Western blotting was performed using anti-phospho-IκB-α, anti-IKK-α, anti-NF-κB (p50, p65), anti-COX-2, anti-iNOS, anti-VCAM-1 and anti-MMP-9 antibodies, respectively.
Results: Sotosaja-hwan inhibited the generation of ONOO<SUP>-</SUP> , NO and ㆍO₂<SUP>-</SUP> in the lipopolysaccharide (LPS)-treated mouse kidney postmitochondrial fraction in vitro. The generation of ONOO<SUP>-</SUP> , NO, ㆍO₂<SUP>-</SUP> and PGE2 were inhibited in the Sotosaja-hwan-administered ob/ob mice groups. The GSH/GSSG ratio was decreased in the ob/ob mice, whereas the ratio was improved in the Sotosaja-hwan-administered groups. Sotosaja-hwan inhibited the protein expression levels of phospho-IκB-α, IKK-α, NF-κB (p50, p65), COX-2, iNOS, VCAM-1 and MMP-9 genes.
Conclusions: These results suggest that Sotosaja-hwan is an effective ONOO<SUP>-</SUP> , ㆍO₂<SUP>-</SUP> and NO scavenger and has NF-κB related anti-inflammatory activity in ob/ob mice. Therefore, Sotosaja-hwan might be a potential therapeutic drug against the inflammation process and inflammation-related diseases.
Methods: Mice were grouped and treated for 5 weeks as follows. Both the normal lean (C57/BL6J black mice) and control obese (ob/ob mice) groups have received standard chow. The experimental groups were fed with a diet of chow supplemented with 30 and 90 mg Sotosaja-hwan per 1 kg of body weight for 14 days. For this study, the fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Western blotting was performed using anti-phospho-IκB-α, anti-IKK-α, anti-NF-κB (p50, p65), anti-COX-2, anti-iNOS, anti-VCAM-1 and anti-MMP-9 antibodies, respectively.
Results: Sotosaja-hwan inhibited the generation of ONOO<SUP>-</SUP> , NO and ㆍO₂<SUP>-</SUP> in the lipopolysaccharide (LPS)-treated mouse kidney postmitochondrial fraction in vitro. The generation of ONOO<SUP>-</SUP> , NO, ㆍO₂<SUP>-</SUP> and PGE2 were inhibited in the Sotosaja-hwan-administered ob/ob mice groups. The GSH/GSSG ratio was decreased in the ob/ob mice, whereas the ratio was improved in the Sotosaja-hwan-administered groups. Sotosaja-hwan inhibited the protein expression levels of phospho-IκB-α, IKK-α, NF-κB (p50, p65), COX-2, iNOS, VCAM-1 and MMP-9 genes.
Conclusions: These results suggest that Sotosaja-hwan is an effective ONOO<SUP>-</SUP> , ㆍO₂<SUP>-</SUP> and NO scavenger and has NF-κB related anti-inflammatory activity in ob/ob mice. Therefore, Sotosaja-hwan might be a potential therapeutic drug against the inflammation process and inflammation-related diseases.
목차
서론
재료 및 방법
성적
고찰
결론
참고문헌
참고문헌 (35)
참고문헌 신청
1. [단행본] - 陳師文 / 1985 / 太平惠民和劑局方 卷五 / 旋風出版社 : 10 ~
2. [학술지(정기간행물)] - Chung HY / 2000 / The inflammatory process in aging / Reviews in Clinical Gerontology 10 : 207 ~ 222
3. [학술지(정기간행물)] - Chung HY / 2001 / The inflammation hypothesis of aging : Molecular modulation by calorie restriction / Ann. N. Y. Acad. Sci 928 : 327 ~ 335
4. [학술지(정기간행물)] - Kim HJ / 2000 / The effect of age on cyclooxygenase-2 gene expression : activation and IκBα degradation / Free Radical Biol Med 28 : 683 ~ 692
5. [학술지(정기간행물)] - Collins T / 1993 / Endotherial nuclear factor-kappa B and the initiation of the atherosclerotic lesion / Lab Invest 68 : 499 ~ 508
2. [학술지(정기간행물)] - Chung HY / 2000 / The inflammatory process in aging / Reviews in Clinical Gerontology 10 : 207 ~ 222
3. [학술지(정기간행물)] - Chung HY / 2001 / The inflammation hypothesis of aging : Molecular modulation by calorie restriction / Ann. N. Y. Acad. Sci 928 : 327 ~ 335
4. [학술지(정기간행물)] - Kim HJ / 2000 / The effect of age on cyclooxygenase-2 gene expression : activation and IκBα degradation / Free Radical Biol Med 28 : 683 ~ 692
5. [학술지(정기간행물)] - Collins T / 1993 / Endotherial nuclear factor-kappa B and the initiation of the atherosclerotic lesion / Lab Invest 68 : 499 ~ 508
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
소토사자환이 ob/ob mouse의 혈당, 고지혈증, Polyol Pathway 및 항산화작용에 미치는 영향
동의생리병리학회지
2007 .01
-
小菟絲子丸이 ob/ob mouse의 혈당, 고지혈증, Polyol Pathway 및 항산화작용에 미치는 영향
동의생리병리학회지
2007 .10
-
Anti-obesity effect of Wild Ginseng extract in ob/ob mice
한국실험동물학회 학술발표대회 논문집
2007 .06
-
오정환(五精丸)이 ob/ob mouse에서 Redox Status 및 NF-${\kappa}B$ Signaling에 미치는 영향
동의생리병리학회지
2008 .01
-
Ob/ob mouse에서 五精丸이 혈당. 고지혈증, Polyol Pathway 및 항산화작용에 미치는 영향
대한한의학회지
2007 .09
-
Anti-Metabolic Syndrome Effects of Wild Ginseng in 3T3-L1 Adipocyte and ob/ob mice
고려인삼학회 학술대회
2009 .05
-
Hypoglycemic activity of KHU-3 in Ob/Ob mice
대한약학회 학술대회
2001 .01
-
Effects of Wild Ginseng on Metabolic Syndrome in 3T3-L1 Adipocyte and ob/ob Mice
한국실험동물학회 학술발표대회 논문집
2009 .08
-
淸血散이 ob/ob mouse의 혈당, 고지혈증, Polyol Pathway 및 Reactive Oxygen Species에 미치는 영향
동의생리병리학회지
2008 .04
-
Anti-diabetic effect of broccoli leaf extract on HepG2 cells and ob/ob mice
한국실험동물학회 학술발표대회 논문집
2020 .07
-
Insulin sensitivity improvement of fermented Korean red ginseng (Panax ginseng) in old aged ob/ob mice
한국실험동물학회 학술발표대회 논문집
2015 .08
-
五精丸이 ob/ob mouse에서 Redox Status 및 NF-κB Signaling에 미치는 영향
동의생리병리학회지
2008 .10
-
Insulin sensitivity improvement of fermented Korean Red Ginseng (Panax ginseng) mediated by insulin resistance hallmarks in old-aged ob/ob mice
Journal of Ginseng Research
2015 .10
-
Anti-obesity Activity of Diglyceride containing Conjugated Linoleic Acid in C57BL/6J ob/ob Mice
한국실험동물학회 학술발표대회 논문집
2009 .04
-
상침자(桑？子)추출물이 ob/ob mouse의 혈당, 혈청 지질, Polyol Pathway, AGE 및 RAGE에 미치는 영향
동의생리병리학회지
2009 .01
-
천연마테차 물추출물의 고지방식이 비만쥐의 체중과 생화학적 변화에 미치는 영향
약학회지
2012 .01
-
Regulation of Dipeptidyl Peptidase (DPP) Ⅳ Activities in Plasma of ob/ob Mice
한국실험동물학회 학술발표대회 논문집
2003 .06
-
청혈산(淸血散)이 Redox Status 및 NF-${\kappa}$B 의존성 단백질에 미치는 영향
동의생리병리학회지
2009 .01
-
Insulin sensitivity improvement of fermented red ginseng (Panax ginseng C.A. Meyer) mediated by insulin resistance hallmarks in old aged ob/ob mice
한국실험동물학회 학술발표대회 논문집
2015 .02
-
Anti-obesity effect of broccoli leaf extract on 3T3-L1 adipocyte and ob/ob mice
한국실험동물학회 학술발표대회 논문집
2020 .07
이 논문의 저자 정보
이 논문과 함께 이용한 논문
노인성 우울증과 알츠하이머병의 관계 : 아밀로이드 이론을 바탕으로
김혜금
,
천은진
,
구본훈
생물치료정신의학
2016
.02
The Peripheral Olfactory System of Tg2576 Mice is Impaired in the Presymptomatic Stage of Alzheimer"s Disease
Seung-Jun Yoo
,
Ji-Hye Lee
,
So Yeun Kim
외 6명
한국실험동물학회 학술발표대회 논문집
2015
.08
87V Scrapie Agent 에 감염된 IM 마우스 뇌조직에서 Sulfated Glycoprotein - 2 ( SGP - 2 ) 의 발현 증가
양형모
,
민본홍
,
최은경
외 1명
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1995
.12
ACM의 알츠하이머 생쥐 모델의 행동과 생체인자에 미치는 영향
김국기
,
최우창
,
김승형
외 5명
동의생리병리학회지
2015
.02
木槿皮가 βA로 유도된 Alzheimer`s Disease 생쥐 모델에 미치는 영향
이상룡
,
정인철
동의생리병리학회지
2004
.06
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2014-519-000284281