지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 135 - 141 (7page)
이용수
초록· 키워드
오류제보하기
A rapid, sensitive and selective analytical method was developed and validated for the determination of compound K, a major intestinal bacterial metabolite of ginsenosides in human plasma. Liquid-liquid extraction was used for sample preparation and analysis, followed by liquid chromatography tandem spectrometric analysis and an electrospray-ionization interface. Compound K was analyzed on a Phenomenex Luna C18 column (100×2.00 mm, 3 μm) with the mobile phase run isocratically with 10 mM ammonium acetate-methanol-acetonitrile (5:47.5:47.5, v/v/v) at a flow rate of 0.5 mL/min. The method was validated for accuracy (relative error <12.63%), precision (coefficient of variation <9.14%), linearity, and recovery. The assay was linear over the entire range of calibration standards i.e., a concentration range of 1 ng/mL to 1,000 ng/ mL (r² >0.9968). The recoveries of compound K after liquid-liquid extraction at 1, 2, 400, and 800 ng/mL were 106.00±0.08%, 103.50±0.19%, 111.45±5.21%, and 89.62±34.46% for intra-day and 85.40±0.08%, 94.50±0.09%, 112.50±5.21%, and 95.87±34.46% for inter-day, respectively. The lower limit of quantification of the analytical method of compound K was 1 ng/ mL in human plasma. The developed method was successfully applied to a pharmacokinetic study of compound K after oral administration in ten of healthy human subjects.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (28)
참고문헌 신청
1. [학술지(정기간행물)] - Attele AS / 1999 / Ginseng pharmacology: multiple constituents and multiple actions / Biochem Pharmacol 58 : 1685 ~ 1693
2. [학술지(정기간행물)] - Hasegawa H / 1996 / Main ginseng saponin metabolites formed by intestinal bacteria / Planta Med 62 : 453 ~ 457
3. [학술지(정기간행물)] - Hasegawa H / 1997 / Role of human intestinal Prevotella oris in hydrolyzing ginseng saponins / Planta Med 63 : 436 ~ 440
4. [학술지(정기간행물)] - Park CS / 2010 / Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases / Appl Microbiol Biotechnol 87 : 9 ~ 19
5. [학술지(정기간행물)] - Jong-Ryul Choi / 2011 / Metabolic Activities of Ginseng and Its Constituents, Ginsenoside Rb_1 and Rg_1, by Human Intestinal Microflora / Journal of Ginseng Research 35 (3) : 301 ~ 307
2. [학술지(정기간행물)] - Hasegawa H / 1996 / Main ginseng saponin metabolites formed by intestinal bacteria / Planta Med 62 : 453 ~ 457
3. [학술지(정기간행물)] - Hasegawa H / 1997 / Role of human intestinal Prevotella oris in hydrolyzing ginseng saponins / Planta Med 63 : 436 ~ 440
4. [학술지(정기간행물)] - Park CS / 2010 / Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases / Appl Microbiol Biotechnol 87 : 9 ~ 19
5. [학술지(정기간행물)] - Jong-Ryul Choi / 2011 / Metabolic Activities of Ginseng and Its Constituents, Ginsenoside Rb_1 and Rg_1, by Human Intestinal Microflora / Journal of Ginseng Research 35 (3) : 301 ~ 307
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2014-524-000316494