지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.12
- 수록면
- 209 - 216 (8page)
이용수
초록· 키워드
오류제보하기
The biological activity of particles is largely dependent on their size in biological systems. Dispersion in the aqueous phase has been both a critical impediment to and a prerequisite for particle studies. Carbon black has been used as a surrogate to investigate the biological effects of carbonaceous particles. Here, biocompatible methods were established to disperse carbon black into ultrafine and fine particles which are generally distinguished by the small size of 100 nm. Carbon black with a distinct particle size, N330 and N990 were suspended in blood plasma, cell culture media, Krebs-Ringer’s solution (KR), or physiological salt solution (PSS). Large clumps were observed in all dispersion preparations; however, sonication improved dispersion - averaged particle sizes for N330 and N990 were 85.0 ± 42.9 and 112.4 ± 67.9 nm, respectively, in plasma; the corresponding sizes in culture media were 84.8 ± 38.4 and 164.1 ± 77.8 nm. However, sonication was not enough to disperse N330 less than 100 nm in either KR or PSS. Application of Tween 80 along with sonication reduced the size of N330 to less than 100 nm, and dispersed N990 larger than 100 nm (73.6 ± 28.8 and 80.1 ± 30.0 nm for N330 and 349.5 ± 161.8 and 399.8 ± 181.1 nm for N990 in KR and PSS, respectively). In contrast, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) exhibited little effect. Electron microscopy confirmed the typical aciniform structure of the carbon arrays; however, zeta potential measurement failed to explain the dispersibility of carbon black. The methods established in this study could disperse carbon black into ultrafine and fine particles, and may serve as a useful model for the study of particle toxicity, particularly size-related effects.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (26)
참고문헌 신청
Ai, J. / 2011 / Nanotoxicology and nanoparticle safety in biomedical designs / Int. J. Nanomed 6:1117~1127
Baan, R. A. / 2007 / Carcinogenic hazards from inhaled carbon black, titanium dioxide, and talc not containing asbestos or asbestiform fibers: recent evaluations by an IARC Monographs Working Group / Inhal. Toxicol 19(1):213~228
Boverhof, D.R / 2010 / Nanomaterial characterization: considerations and needs for hazard assessment and safety evaluation / Anal. Bioanal. Chem 396:953~961
Brook, R. D. / 2010 / Particulate matter air pollution and cardiovascular disease: An update to the scientific statement from the American Heart Association / Circulation 121:2331~2378
Castranova, V. / 2011 / Overview of current toxicological knowledge of engineered nanoparticles / J. Occup. Environ. Med 53:14~17
Baan, R. A. / 2007 / Carcinogenic hazards from inhaled carbon black, titanium dioxide, and talc not containing asbestos or asbestiform fibers: recent evaluations by an IARC Monographs Working Group / Inhal. Toxicol 19(1):213~228
Boverhof, D.R / 2010 / Nanomaterial characterization: considerations and needs for hazard assessment and safety evaluation / Anal. Bioanal. Chem 396:953~961
Brook, R. D. / 2010 / Particulate matter air pollution and cardiovascular disease: An update to the scientific statement from the American Heart Association / Circulation 121:2331~2378
Castranova, V. / 2011 / Overview of current toxicological knowledge of engineered nanoparticles / J. Occup. Environ. Med 53:14~17
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UCI(KEPA) : I410-ECN-0101-2014-513-000614574