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논문 기본 정보

자료유형
학술대회자료
저자정보
Shiro Ikeda (Tokai University) Masafumi Oikawa (Tokai University) Yoshika Sekine (Tokai University)
저널정보
제어로봇시스템학회 제어로봇시스템학회 국제학술대회 논문집 ICCAS-SICE 2009
발행연도
2009.8
수록면
4,486 - 4,489 (4page)

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초록· 키워드

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Bioassay, which detects biohazardous property of environmental contaminants, is still being an unexploredfield of air quality monitoring. This study aims to develop a simple testing system of toxicity of the indoor particulate matter by detecting bioluminescence reduction of marine bacterium Vibrio fischeri. Suspended particulate matter in indoor and outdoor air was collected on a quartz fiber filter at a flow rate of 23.5 L/min. by a low volume air sampler for 7-day duration, at the terrace and living room of an apartment house in Kanagawa, Japan. Collected materials wereextracted with sterilized distilled water by mild shaking. After filtration with 0.45 ??m pore size filter, the extracts were subsequently mixed with a bacterium solution in a well of 24-well plate and time courses of bioluminescence intensity were measured by a luminometer. As a result, the water extracts of indoor and outdoor aerosol samples significantly reduced the bioluminescence of Vibrio fischeri and the inhibition per sampling volume increased with increasing TSPconcentrations. The inhibition per unit mass of the indoor aerosols became greater than that of outdoor aerosols, whenincreasing in air change rate. The inhibition tended to correlate with nitrate and sulfate concentrations in the extracts. This study showed possible application of bioassay to the monitoring of indoor suspended particle contamination.

목차

Abstract
1. INTRODUCTION
2. EXPERIMENTAL
3. RESULT AND DISCUSSION
4. CONCLUSION
ACKNOWLEDGEMENT
REFERENCES

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