Immediately transcripted genes in various hepatic ischemia models

Kang Kook Choi; Jin A Cho; Se Hoon Kim; Sang Woo Lee; Seon Ok Min; Kyung Sik Kim

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자료유형: 학술저널

저자정보: Kang Kook Choi (Samsung Medical Center, Sungkyunkwan University School of Medicine) Jin A Cho (Gachon University of Medicine and Science) Se Hoon Kim (Yonsei University College of Medicine) Sang Woo Lee (Graduate School of Yonsei University) Seon Ok Min (Graduate School of Yonsei University) Kyung Sik Kim (Yonsei University College of Medicine)

저널정보: 대한외과학회 Annals of Surgical Treatment and Research Journal of the Korean Surgical Society Vol.83 No.5

발행연도: 2012.11

수록면: 298 - 306 (9page)

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Purpose: To elucidate the characteristic gene transcription profiles among various hepatic ischemia conditions, immediately transcribed genes and the degree of ischemic injury were compared among total ischemia (TI), intermittent clamping (IC), and ischemic preconditioning (IPC). Methods: Sprague-Dawley rats were equally divided into control (C, sham-operated), TI (ischemia for 90 minutes), IC (ischemia for 15 minutes and reperfusion for 5 minutes, repeated six times), and IPC (ischemia for 15 minutes, reperfusion for 5 minutes, and ischemia again for 90 minutes) groups. A cDNA microarray analysis was performed using hepatic tissues obtained by partial hepatectomy after occluding hepatic inflow. Results: The cDNA microarray revealed the following: interleukin (IL)-1β expression was 2-fold greater in the TI group than in the C group. In the IC group, IL-1α/β expression increased by 2.5-fold, and Na＋/K＋ ATPase β1 expression decreased by 2.4-fold. In the IPC group, interferon regulatory factor-1, osteoprotegerin, and retinoblastoma-1 expression increased by approximately 2-fold compared to that in the C group, but the expression of Na＋/K＋ ATPase β1 decreased 3-fold. Conclusion: The current findings revealed characteristic gene expression profiles under various ischemic conditions. However, additional studies are needed to clarify the mechanism of protection against IPC.

#Reperfusion injury #Ischemic preconditioning #Necrosis #Apoptosis #Microarray analysis

참고문헌 (30)

참고문헌 신청

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