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논문 기본 정보

자료유형
학술저널
저자정보
Yu-Jin Kim (Kyung Hee University) Ok Ran Lee (Kyung Hee University) Kyung-Tack Kim (Korea Food Research Institute) Deok-Chun Yang (Kyung Hee University)
저널정보
고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.36 No.4
발행연도
2012.10
수록면
442 - 448 (7page)

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초록· 키워드

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Somatic embryogenesis is one of good examples of the basic research for plant embryo development as well as an important technique for plant biotechnology such as medicinally important plants. Single embryos develop into normal plantlets with shoots and roots. Therefore, direct single embryogenesis derived from single cells is highly important for normal plant regeneration. Here we demonstrate that the cyclic secondary somatic embryogenesis in Panax ginseng Meyer is a permanent source of embryogenic material that can be used for genetic manipulations. Secondary somatic embryos were originated directly from the primary somatic embryos on hormone-free Murashige and Skoog medium, and proliferated further in a cyclic manner. EM medium (one third of modifi ed MS medium [MS medium containing half amount of NH4NO3 and KNO3] with 2% to 3% sucrose) favored further development of proliferated secondary somatic embryos into plantlets with root system. The plantlets developed into plants with well-developed taproots in half-strength Schenk and Hildebrandt basal medium supplemented with 0.5% activated charcoal.

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INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

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UCI(KEPA) : I410-ECN-0101-2014-524-001184512