지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.9
- 수록면
- 218 - 226 (9page)
이용수
초록· 키워드
오류제보하기
Dopamine is an enteric neurotransmitter that regulates gastrointestinal motility. This study was done to investigate whether dopamine modulates spontaneous pacemaker activity in cultured interstitial cells of Cajal (ICCs) from mouse using whole cell patch clamp technique, RT-PCR and live Ca<SUP>2+</SUP> imaging analysis. ICCs generate pacemaker inward currents at a holding potential of -70 mV and generate pacemaker potentials in current-clamp mode. Dopamine did not change the frequency and amplitude of pacemaker activity in small intestinal ICCs. On the contrary dopamine reduced the frequency and amplitude of pacemaker activity in large intestinal ICCs. RT-PCR analysis revealed that Dopamine2 and 4-receptors are expressed in c-Kit positive ICCs. Dopamine2 and 4 receptor agonists inhibited pacemaker activity in large intestinal ICCs mimicked those of dopamine. Domperidone, dopamine2 receptor antagonist, increased the frequency of pacemaker activity of large intestinal ICCs. In Ca<SUP>2+</SUP>-imaging, dopamine inhibited spontaneous intracellular Ca<SUP>2+</SUP> oscillations of ICCs. These results suggest that dopamine can regulate gastrointestinal motility through modulating pacemaker activity of large intestinal ICCs and dopamine effects on ICCs are mediated by dopamine2 receptor and intracellular Ca<SUP>2+</SUP> modulation.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
Acknowledgements
REFERENCES
참고문헌 (30)
참고문헌 신청
Abe M / 1984 / Hypertensive crisis induced by metocloprimed in patient with pheochromocytoma / Angiology 35:122~128
Adler-Graschinsky E / 1984 / Metoclopramide increases the release of catecholamines from isolated human phaeochromocytomas / J Hypertens 2:127~129
Eaker EY / 1988 / Dopamine and norepinephrine in the gastrointestinal tract of mice and the effects of neurotoxins / J Pharmcol Exp Ther 244:438~442
Gershon MD / 1967 / Inhibition of gastrointestinal movement by sympathetic nerve stimulation: the site of action / J Physiol (Lond) 198:317~329
Grivegnee AR / 1984 / Effect of dopamine on dog distal colon in-vitro / J Pharm Pharmacol 36:454~457
Adler-Graschinsky E / 1984 / Metoclopramide increases the release of catecholamines from isolated human phaeochromocytomas / J Hypertens 2:127~129
Eaker EY / 1988 / Dopamine and norepinephrine in the gastrointestinal tract of mice and the effects of neurotoxins / J Pharmcol Exp Ther 244:438~442
Gershon MD / 1967 / Inhibition of gastrointestinal movement by sympathetic nerve stimulation: the site of action / J Physiol (Lond) 198:317~329
Grivegnee AR / 1984 / Effect of dopamine on dog distal colon in-vitro / J Pharm Pharmacol 36:454~457
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UCI(KEPA) : I410-ECN-0101-2014-510-001368821