지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.10
- 수록면
- 229 - 234 (6page)
이용수
초록· 키워드
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Purpose: Trauma-induced suppression of cellular immune function likely contributes to sepsis, multiple organ dysfunction syndrome and death. T cell proliferation decreases after traumatic stress. The addition of prostaglandin E2 (PGE2), which depresses immune function after hemorrhage and trauma, to T-cells decreases T-cell proliferation; and hypertonic saline restores PGE2-induced T-cell suppression. Recently, it has become apparent that macrophage migration inhibitory factor (MIF) plays a central role in several immune responses, including T-cell proliferation. However, the role of MIF in mediating hypertonic saline (HTS) restoration of T cell dysfunction is unknown. Therefore, we hypothesize that T cell immune restoration by HTS occurs, at least in part, by a MIF-mediated mechanism. Methods: Jurkat cells were cultured in Roswell Park Memorial Institute media, at a final concentration of 2.5 × 106 cell/mL. The effects of HTS on T-cell proliferation following PGE2-induced suppression were evaluated in Jurkat cells: HTS at 20 or 40 mmol/L above isotonicity was added. MIF levels were determined by enzyme-linked immunosorbent assay and western blot analysis. Results: PGE2 caused a 15.0% inhibition of Jurkat cell proliferation, as compared to the control. MIF levels decreased in PGE2-suppressed cells, as compared to the control. MIF levels were higher in cells treated with HTS than PGE2-stimulated cells. Conclusion: The role of HTS in restoring Jurkat cells proliferation suppressed by PGE2, at least in part, should be mediated through a MIF pathway.
목차
Purpose
INTRODUCTION
METHODS
RESULTS
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (25)
참고문헌 신청
1. [학술지(정기간행물)] - Napolitano LM / 1999 / Immune dysfunction in trauma / Surg Clin North Am 79 : 1385 ~ 1416
2. [학술지(정기간행물)] - Coimbra R / 1995 / Hypertonic/hyperoncotic fluids reverse prostaglandin E2 (PGE2)-induced T-cell suppression / Shock 4 : 45 ~ 49
3. [학술지(정기간행물)] - Bacher M / 1996 / An essential regulatory role for macrophage migration inhibitory factor in T-cell activation / Proc Natl Acad Sci U S A 93 : 7849 ~ 7854
4. [학술지(정기간행물)] - Bernhagen J / 1998 / Regulation of the immune response by macrophage migration inhibitory factor: biological and structural features / J Mol Med (Berl) 76 : 151 ~ 161
5. [학술지(정기간행물)] - Lee JY / 2004 / Normal serum macrophage migration inhibitory factor concentrations of Korean people according to age / J Korean Geriatr Soc 8 : 165 ~ 169
2. [학술지(정기간행물)] - Coimbra R / 1995 / Hypertonic/hyperoncotic fluids reverse prostaglandin E2 (PGE2)-induced T-cell suppression / Shock 4 : 45 ~ 49
3. [학술지(정기간행물)] - Bacher M / 1996 / An essential regulatory role for macrophage migration inhibitory factor in T-cell activation / Proc Natl Acad Sci U S A 93 : 7849 ~ 7854
4. [학술지(정기간행물)] - Bernhagen J / 1998 / Regulation of the immune response by macrophage migration inhibitory factor: biological and structural features / J Mol Med (Berl) 76 : 151 ~ 161
5. [학술지(정기간행물)] - Lee JY / 2004 / Normal serum macrophage migration inhibitory factor concentrations of Korean people according to age / J Korean Geriatr Soc 8 : 165 ~ 169
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UCI(KEPA) : I410-ECN-0101-2013-514-002683698