지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 7 - 16 (10page)
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초록· 키워드
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Purpose: The aim of this study was to find the dose of agonistic 4-1BB monoclonal antibody (㎃b) that results in optimal T cell activation.
Methods: Cancer was induced in mice by an intrahepatic parenchymal injection of 1×10? cells of CT26 cells. Cancer-carrying mice (n=84) were divided into seven groups and treated with either rat IgG or agonistic 4-1BB monoclonal antibody (㎃b) (5㎍, 10㎍, 20㎍, 100㎍, 200㎍, or 300㎍). All treatments were administered intraperitoneally on days 7, 9, and 11. Mice from each group were sacrificed on days 14, 28, and 42. Harvested livers were weighed and the numbers of T cells in the splenocytes were analyzed with a FACS Vantage flow cytometer.
Results: Liver weights increased when 5㎍ of agonistic 4-1BB ㎃b was administered, but showed no additional weight increase for doses greater than 10㎍. The absolute numbers of CD4＋ and CD8＋ T cells increased in groups treated with low doses of agonistic 4-1BB ㎃b (5㎍, 10㎍, or 20㎍), but did not increase in the groups treated with high doses of ㎃b (100㎍, 200㎍, or 300㎍). The levels of CD4/annexin V and CD8/annexin V increased as the dose increased, and the absolute cell numbers of CD4/annexin V were greater than those of CD8/annexin V.
Conclusion: Liver weight, including the cancer mass, failed to increase at agonistic 4-1BB ㎃b doses greater than 10㎍. A high dose (≥100㎍) of agonistic 4-1BB ㎃b resulted in lower counts of absolute T cells. This study suggests that a low dose (20㎍) of agonistic 4-1BB ㎃b can be used for optimal T cell activation in combination with other anti-cancer treatments.
Methods: Cancer was induced in mice by an intrahepatic parenchymal injection of 1×10? cells of CT26 cells. Cancer-carrying mice (n=84) were divided into seven groups and treated with either rat IgG or agonistic 4-1BB monoclonal antibody (㎃b) (5㎍, 10㎍, 20㎍, 100㎍, 200㎍, or 300㎍). All treatments were administered intraperitoneally on days 7, 9, and 11. Mice from each group were sacrificed on days 14, 28, and 42. Harvested livers were weighed and the numbers of T cells in the splenocytes were analyzed with a FACS Vantage flow cytometer.
Results: Liver weights increased when 5㎍ of agonistic 4-1BB ㎃b was administered, but showed no additional weight increase for doses greater than 10㎍. The absolute numbers of CD4＋ and CD8＋ T cells increased in groups treated with low doses of agonistic 4-1BB ㎃b (5㎍, 10㎍, or 20㎍), but did not increase in the groups treated with high doses of ㎃b (100㎍, 200㎍, or 300㎍). The levels of CD4/annexin V and CD8/annexin V increased as the dose increased, and the absolute cell numbers of CD4/annexin V were greater than those of CD8/annexin V.
Conclusion: Liver weight, including the cancer mass, failed to increase at agonistic 4-1BB ㎃b doses greater than 10㎍. A high dose (≥100㎍) of agonistic 4-1BB ㎃b resulted in lower counts of absolute T cells. This study suggests that a low dose (20㎍) of agonistic 4-1BB ㎃b can be used for optimal T cell activation in combination with other anti-cancer treatments.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
CONCLUSION
REFERENCES
참고문헌 (31)
참고문헌 신청
1. [학술지(정기간행물)] - Vinay DS / 2006 / Dual immunoregulatory pathways of 4-1BB signaling / J Mol Med 84 : 726 ~ 736
2. [학술지(정기간행물)] - Greenwald RJ / 2005 / The B7 family revisited / Annu Rev Immunol 23 : 515 ~ 548
3. [학술지(정기간행물)] - Watts TH / 2005 / TNF/TNFR family members in costimulation of T cell responses / Annu Rev Immunol 23 : 23 ~ 68
4. [학술지(정기간행물)] - Cheuk AT / 2004 / Role of 4-1BB:4-1BB ligand in cancer immunotherapy / Cancer Gene Ther 11 : 215 ~ 226
5. [학술지(정기간행물)] - Kwon BS / 1994 / Genomic organization and chromosomal localization of the T-cell antigen 4-1BB / J Immunol 152 : 2256 ~ 2262
2. [학술지(정기간행물)] - Greenwald RJ / 2005 / The B7 family revisited / Annu Rev Immunol 23 : 515 ~ 548
3. [학술지(정기간행물)] - Watts TH / 2005 / TNF/TNFR family members in costimulation of T cell responses / Annu Rev Immunol 23 : 23 ~ 68
4. [학술지(정기간행물)] - Cheuk AT / 2004 / Role of 4-1BB:4-1BB ligand in cancer immunotherapy / Cancer Gene Ther 11 : 215 ~ 226
5. [학술지(정기간행물)] - Kwon BS / 1994 / Genomic organization and chromosomal localization of the T-cell antigen 4-1BB / J Immunol 152 : 2256 ~ 2262
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UCI(KEPA) : I410-ECN-0101-2013-514-002680162