지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.6
- 수록면
- 343 - 349 (7page)
이용수
초록· 키워드
오류제보하기
Purpose: This study demonstrates that pharmacologic induction of heme oygenase-1 (HO-1) along with catalytic activation significantly modulated apoptosis of Jurkat cells induced by mycophenolic acid (MPA).
Methods: Cells were cultured with the presence or absence of MPA. Flow cytometric analysis was performed after propidium iodide staining. Western blotting of HO-1, Bcl, and Bax was also performed. Cells were stained 4’-6-Diamidino-2-phenylindole (DAPI) and measured by flow cytometry in the absence or presence of CoPPIX. Results: Treatment of MPA decreased cell viability in a dose- and time-dependent manner. MPA-induced cell death was confirmed as apoptosis characterized by sub G0/G1 phase arrest. Expression of HO-1 assumes a pattern of decline after rising at the initial phase. CoPPIX, HO-1 inducer, significantly inhibited the cisplatin-induced apoptosis. Treatment of MPA resulted in reactive oxygen species (ROS) generation in Jurkat cells. CoPPIX attenuated ROS production in MPA-treated cells.
Conclusion: This result suggests that the protective mechanism of HO-1 on MPA-induced cytotoxicity is associated with direct inhibition of ROS generation and mitochondrial permeability transition.
Methods: Cells were cultured with the presence or absence of MPA. Flow cytometric analysis was performed after propidium iodide staining. Western blotting of HO-1, Bcl, and Bax was also performed. Cells were stained 4’-6-Diamidino-2-phenylindole (DAPI) and measured by flow cytometry in the absence or presence of CoPPIX. Results: Treatment of MPA decreased cell viability in a dose- and time-dependent manner. MPA-induced cell death was confirmed as apoptosis characterized by sub G0/G1 phase arrest. Expression of HO-1 assumes a pattern of decline after rising at the initial phase. CoPPIX, HO-1 inducer, significantly inhibited the cisplatin-induced apoptosis. Treatment of MPA resulted in reactive oxygen species (ROS) generation in Jurkat cells. CoPPIX attenuated ROS production in MPA-treated cells.
Conclusion: This result suggests that the protective mechanism of HO-1 on MPA-induced cytotoxicity is associated with direct inhibition of ROS generation and mitochondrial permeability transition.
목차
서론
방법
결과
고찰
결론
REFERENCES
참고문헌 (14)
참고문헌 신청
1. [학술지(정기간행물)] - Florey HW / 1946 / Mycophenolic acid: an antibiotic from penicillium brevicompactum Dierckx / Lancet 247 : 46 ~ 49
2. [학술지(정기간행물)] - Allison AC / 1993 / Mechanisms of action of mycophenolic acid / Ann N Y Acad Sci 696 : 63 ~ 87
3. [학술지(정기간행물)] - Allison AC / 2000 / Mycophenolate mofetil and its mechanisms of action / Immunopharmacology 47 : 85 ~ 118
4. [학술지(정기간행물)] - Natsumeda Y / 1993 / Human type I and II IMP dehydrogenases as drug targets / Ann N Y Acad Sci 696 : 88 ~ 93
5. [학술지(정기간행물)] - Konno Y / 1991 / Expression of human IMP dehydrogenase types I and II in Escherichia coli and distribution in human normal lymphocytes and leukemic cell lines / J Biol Chem 266 : 506 ~ 509
2. [학술지(정기간행물)] - Allison AC / 1993 / Mechanisms of action of mycophenolic acid / Ann N Y Acad Sci 696 : 63 ~ 87
3. [학술지(정기간행물)] - Allison AC / 2000 / Mycophenolate mofetil and its mechanisms of action / Immunopharmacology 47 : 85 ~ 118
4. [학술지(정기간행물)] - Natsumeda Y / 1993 / Human type I and II IMP dehydrogenases as drug targets / Ann N Y Acad Sci 696 : 88 ~ 93
5. [학술지(정기간행물)] - Konno Y / 1991 / Expression of human IMP dehydrogenase types I and II in Escherichia coli and distribution in human normal lymphocytes and leukemic cell lines / J Biol Chem 266 : 506 ~ 509
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Mycophenolic Acid가 사람 Jurkat 세포의 자멸사에서 활성 산소 생성에 미치는 영향
Annals of Surgical Treatment and Research
2008 .09
-
Mycophenolic acid mediated mitochondrial membrane potential transition change lead to T lymphocyte apoptosis
Annals of Surgical Treatment and Research
2011 .10
-
FK506이 T 림프구 사멸에서 활성산소 생성에 미치는 영향
Annals of Surgical Treatment and Research
2009 .11
-
FK506에 의한 Jurkat 세포자멸사 유전자 발현분석
Annals of Surgical Treatment and Research
2009 .10
-
CD29 및 CD98 활성 매개에 의한 Jurkat T 세포의 유착과 그 활용
약학회지
2009 .01
-
폐암세포주에서 Heme Oxygenase-1의 역할
Tuberculosis and Respiratory Diseases
2006 .01
-
鷄血藤 추출물이 Jurkat T 임파구의 세포고사 및 세포주기 억제에 미치는 효과
동의생리병리학회지
2004 .02
-
Ginsenoside Ro inhibits LPS-induced inflammation via Up-regulating HO-1 in RAW 264.7 Cells
한국실험동물학회 학술발표대회 논문집
2013 .08
-
Effects of Hyperbaric Pressure on Cellular Morphology, Proliferation and Protein Expression of Jurkat Cell
Molecular & cellular toxicology
2005 .01
-
Heme Oxygenase-l Induction by YS 51, An Analogue of Higenamine, Inhibits Nitric Oxide-Induced Apoptosis of ROS 17/2.8 cells
대한약학회 학술대회
2005 .01
-
Mycophenolic Acid가 올레산으로 유도한 흰쥐 혈관 평활근 세포의 증식에 미치는 영향
Annals of Surgical Treatment and Research
2007 .03
-
Up-regulation of Heme Oxygenase-1 Expression by cAMP-elevating Agents in RAW 264.7 cells
The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology
2002 .01
-
The neuroprotective effect of mycophenolic acid via anti-apoptosis in perinatal hypoxic-ischemic brain injury
Clinical and Experimental Pediatrics
2007 .01
-
Molt-4 T 세포에 있어서 Mycophenolic Acid 에 의한 세포고사의 신호전달 기전
Annals of Surgical Treatment and Research
2002 .01
-
Jurkat T 임파구의 세포주기 기전에 미치는 저근백피(Ailanthus altissima)의 효과
약학회지
2002 .01
-
FK506의 T 림프구 세포 독성 기전
Annals of Surgical Treatment and Research
2007 .09
-
Heme oxygenase-1 is a key molecule underlying differential response of TW37-induced apoptosis in human mucoepidermoid carcinoma cells
한국실험동물학회 학술발표대회 논문집
2019 .07
-
Jurkat T 세포에 있어서 Mizoribine에 의한 세포고사의 신호전달기전 연구
Annals of Surgical Treatment and Research
2004 .04
-
Oxidative stress of silver nanoparticles in Jurkat cells
환경독성보건학회 심포지엄 및 학술대회
2009 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0