지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Streptomycetes are gram-positive filamentous bacteria that are well-known for producing a vast array of bioactive compounds, including more than 70 % of commercially important antibiotics. For the research about Streptomyces sp., the protoplast and electroporation transformation method have been the general techniques for the construction of transformants. However, these techniques have low efficiency and are time-consuming. Another option is intergenic conjugation, which is used for DNA transfer using methylation-deficient E. coli as a DNA donor to avoid the methylated- DNA-dependent restriction systems of actinomycetes. This conjugation method has been widely improved and applied to many other actinomycetes. In this research, an effective transformation procedure for the construction of expression vector by using gateway system was established to avoid limit of restriction enzyme site for cloning of target gene based on transconjugation by Escherichia coli ET12567/pUZ8002 with a pSET152 integration vector.
목차
서론
재료 및 방법
결과
고찰
REFERENCES
참고문헌 (0)
참고문헌 신청이 논문의 저자 정보
이 논문과 함께 이용한 논문
Effects of the Photic Stimulation on Electroencephalogram in Pediatric Epilepsy Patients
Joong Soo Yoon
,
Hyun Ju Choi
대한의생명과학회지
2012
.12
A Hydrogel Film Containing Propolis Nanoparticles as a Wound Healing Membrane
Jin Kim
,
Yong Moon Kim
,
Dong-Woon Kim
외 1명
대한의생명과학회지
2012
.06
Clinical Evaluation of Human Papillomavirus DNA Genotyping Assay to Diagnose Women Cervical Cancer
Sunghyun Kim
,
Dongsup Lee
,
Yeun Kim
외 8명
대한의생명과학회지
2012
.06
사람과 미래환경
강미아
한국환경정책학회 학술대회논문집
2016
.02
휜의 피치 및 배열 방식에 따른 프리히터의 전열 성능에 관한 연구
유지훈
,
김귀순
한국유체기계학회 논문집
2013
.12
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2013-510-001743940